Protein Info for BWI76_RS17535 in Klebsiella michiganensis M5al

Annotation: rRNA metabolism protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1034 TIGR03315: putative selenate reductase, YgfK subunit" amino acids 2 to 1029 (1028 residues), 1669.6 bits, see alignment E=0 PF14691: Fer4_20" amino acids 455 to 534 (80 residues), 59.9 bits, see alignment 8.6e-20 PF07992: Pyr_redox_2" amino acids 554 to 841 (288 residues), 133.7 bits, see alignment E=3.7e-42 PF01494: FAD_binding_3" amino acids 554 to 586 (33 residues), 23.2 bits, see alignment (E = 1.5e-08) PF00890: FAD_binding_2" amino acids 555 to 589 (35 residues), 23.4 bits, see alignment (E = 1.3e-08) PF13450: NAD_binding_8" amino acids 557 to 595 (39 residues), 36.2 bits, see alignment (E = 2.4e-12)

Best Hits

Swiss-Prot: 80% identical to YGFK_ECOLI: Putative oxidoreductase YgfK (ygfK) from Escherichia coli (strain K12)

KEGG orthology group: K12527, putative selenate reductase [EC: 1.97.1.9] (inferred from 86% identity to cro:ROD_33961)

Predicted SEED Role

"Predicted oxidoreductase, Fe-S subunit"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5L9 at UniProt or InterPro

Protein Sequence (1034 amino acids)

>BWI76_RS17535 rRNA metabolism protein (Klebsiella michiganensis M5al)
MGDIMRPVPFEELLTRIFDEYQSQRSIFGIPEQQFYSPQAQRSIGVFGESCATPLGPAAG
PHTQLAQNIITAWLTGGRFIELKTVQILDRLELEKPCIDAEDECFNTEWSTEFTLKKAWD
EYLKAWFALHLLEQVFPLATNKESKSFIFNMSVGYNLDGIKQPPMQEFIDNMMDASAHPK
FAQYRDTLNRWLQNKPFIDSLPTCARKDGLDNLAQRVPARLVQGVTLSTMHGCPPDEIEA
ICRYMLTEKNLNTFVKLNPTLLGYPRVRQILDACGFGYIGLSEASFDHDLKIDRALEMLT
RLMALAKERSLGFGVKLTNTLGTVNNKGALPGDEMYMSGRALFPLSINVAALLSRAFDGK
LPISYSGGASQLNIREIFDTGIRPITMATDLLKPGGYLRLSECMRELEKADGWNMTQIDV
ERLNALAEKAVSMDYTQKQWKPEERIDTGEPLPLLDCYVAPCVTACAIKQDIPEYIRLLG
EHRYADALELIYQRNALPAITGHICDHQCQYNCTRLDYDSALNIRELKKVALEKGWDAYR
SRWHKPAGSGSRHPVAVIGAGPAGLAAGYFLARAGHPVTVFEREANAGGVVQNIIPQFRI
PGELIQHDIDFVVAHGVKIEYGCDPHLSVEKLQAKGFRYVLVGTGTDKNSGVKLGGDNQN
VHKSLQFLRQFNRGAELNLGKHVAVVGAGNTAMDCARAALRVPGVQSATIVYRRSQQEMP
AWREEYDEALLDGVGFEWLCNPEQFNADGTLVVRVMKLGEPDEKGRRRPVETDELRTLKV
DSLITAIGEQQDGEALSAMGIPLDPQGWPVVNADGETSKPNVFLIGDVQRGPSSIVSAIG
NARRATDVILARENIASRYGNKVWNNVDPAKVYQRKGAIAVTLVDKNQREAFVEQEASRC
LECNYVCSKCVDVCPNRANISVAVPGFQNRFQTLHLDAYCNECGNCAQFCPWQGKPYKDK
ITVFSLEQDFVNSTNPGFFVAGASVKVRQDDQTWQLEINDRGQFNEVPAQLDAMCRIISH
IHQHQSYLLGGVEV