Protein Info for BWI76_RS17515 in Klebsiella michiganensis M5al

Annotation: dihydropyrimidinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 TIGR02033: dihydropyrimidinase" amino acids 3 to 453 (451 residues), 624.8 bits, see alignment E=4.6e-192 PF01979: Amidohydro_1" amino acids 50 to 434 (385 residues), 80.9 bits, see alignment E=1.1e-26

Best Hits

Swiss-Prot: 79% identical to PHYDA_ECO27: D-phenylhydantoinase (hyuA) from Escherichia coli O127:H6 (strain E2348/69 / EPEC)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 90% identity to cro:ROD_34001)

MetaCyc: 79% identical to phenylhydantoinase (Escherichia coli K-12 substr. MG1655)
3.5.2.-

Predicted SEED Role

"Hypothetical hydrolase YgeZ (EC 3.-.-.-)" (EC 3.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.-.-.-

Use Curated BLAST to search for 3.-.-.- or 3.5.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5H3 at UniProt or InterPro

Protein Sequence (468 amino acids)

>BWI76_RS17515 dihydropyrimidinase (Klebsiella michiganensis M5al)
MRVLIKNGLVVNADGQVKQDLLIEDGLVKRLADSIEAEQPCEVIDAEGCYVMPGGVDVHT
HFNIDVGLARSCDDFFTGTRAAACGGTTTIVDHMGFGPVGCHLRHQFEVYRGYAAYKAVI
DYSFHGVIQHVNHGILDEIPMMVDSGISSFKLYLTYQYKLNDDEILQAMRHLQRAGALTT
VHPENDAAIAQRRAEFINAGKTAPRYHALSRPLECEAEAIARTINLAQLAGNAPLYIVHL
SNALGLEYLRLARRRRQPVWVETCPQYLLLDERCYEREDALNYLLSPPLRNASNNDALWS
GISNGDIDTVATDHCAFSAQQRRQISGGDFSRCPNGLPGVENRLLLLFSHGVMTGRITPE
RFVALTSANPAKLFGLWPQKGLLAPGADGDVVIIDPKRTTTIRHDMLHDNADYSPYEGFI
CQGAIRQTLSHGRVIFDNGTFSGTAGQGRFLRRQPFSTAHLAVSGTET