Protein Info for BWI76_RS17480 in Klebsiella michiganensis M5al

Annotation: aminoacyl-tRNA hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 97% identical to PTH_KLEP7: Peptidyl-tRNA hydrolase (pth) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01056, peptidyl-tRNA hydrolase, PTH1 family [EC: 3.1.1.29] (inferred from 97% identity to kpn:KPN_02242)

MetaCyc: 92% identical to peptidyl-tRNA hydrolase (Escherichia coli K-12 substr. MG1655)
Aminoacyl-tRNA hydrolase. [EC: 3.1.1.29]

Predicted SEED Role

"Peptidyl-tRNA hydrolase (EC 3.1.1.29)" (EC 3.1.1.29)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5L3 at UniProt or InterPro

Protein Sequence (194 amino acids)

>BWI76_RS17480 aminoacyl-tRNA hydrolase (Klebsiella michiganensis M5al)
MSIKLIVGLANPGAEYAATRHNAGAWYVDLLAERHRAPLREESKFFGYTSRINLAGEDVR
LLVPTTFMNLSGKAVGAMATFFRINPDEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDI
ISKLGNNPNFHRLRVGIGHPGDKNKVVGFVLGKPPASEQKLIDEAVDEAARCTEVWLKDG
LTKATNRLHAFKAQ