Protein Info for BWI76_RS17365 in Klebsiella michiganensis M5al

Updated annotation (from data): Nitrite reductase (NAD(P)H) (EC 1.7.1.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrite reductase (NAD(P)H)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1356 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 4 to 275 (272 residues), 191.4 bits, see alignment E=1.5e-59 amino acids 405 to 691 (287 residues), 167.3 bits, see alignment E=3.5e-52 PF13738: Pyr_redox_3" amino acids 69 to 243 (175 residues), 44.1 bits, see alignment E=1.1e-14 PF00070: Pyr_redox" amino acids 143 to 212 (70 residues), 51.5 bits, see alignment 8e-17 amino acids 547 to 620 (74 residues), 58.3 bits, see alignment 6e-19 PF18267: Rubredoxin_C" amino acids 309 to 371 (63 residues), 43.7 bits, see alignment 1.4e-14 amino acids 717 to 786 (70 residues), 35.4 bits, see alignment 5.6e-12 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 405 to 1198 (794 residues), 1093.7 bits, see alignment E=0 PF04324: Fer2_BFD" amino acids 821 to 868 (48 residues), 43.6 bits, see alignment (E = 2e-14) PF03460: NIR_SIR_ferr" amino acids 957 to 1020 (64 residues), 44.6 bits, see alignment 6.5e-15 PF01077: NIR_SIR" amino acids 1029 to 1165 (137 residues), 78.6 bits, see alignment E=2.4e-25 PF13806: Rieske_2" amino acids 1244 to 1344 (101 residues), 100.9 bits, see alignment 2.4e-32 TIGR02378: nitrite reductase [NAD(P)H], small subunit" amino acids 1244 to 1345 (102 residues), 116.4 bits, see alignment 5.9e-38

Best Hits

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5M8 at UniProt or InterPro

Protein Sequence (1356 amino acids)

>BWI76_RS17365 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (Klebsiella michiganensis M5al)
MTRQLVIIGNGMAATRLAQALVERDARRFSITIIGDEPRQAYNRIQLSPVLGAEKTAGAT
RLLPAEWYSQHNVTVRAGETVTAVDMAARTLQTTAGELGWDELVFATGSLPFLPPLPGIT
LPHVFAFRTLADVEGILAIDGPAVVIGGGVLGVEAAAALRRHGDSVTLLHRGSWLMEQQT
DAFVGEQLQMLLAERGIGCVMESRIAAIDEHQVLLEDGRAFAASRVVLATGVQPNKRLAE
RSGLACGRGILVDRRLATAQPGVSALGECCEIDGQTWGLVAPCLRQAEVLADRLCAIPGE
DFRWQDSGTRLKVTDIELFSAGELRADEQDDVYTSWDPLDRHYRRLLLRDGKLRGVLLLG
DCSSAAPLTALLGASAPAPAEWLFDPSSTMQPRAVGQTTMTKPVLVLVGHGMVGHHFLEQ
CVSRDLHQQYRIVVFCEERYAAYDRVHLTEYFAGRSAESLSLVEGDFFTQHGIELRLSES
VASIDREARVVRDAFGHETHWDKLVLATGSYPFVPPVPGHNLEGCFVYRTLDDLDQIAAR
AATARRGVVIGGGLLGLEAANALKQLGLETHVVEFAPNLMAVQLDNGGAAMLREKISELG
VGVHTSKATTEIVRNEQGLQLNFADGSSLATDMLVFSAGIRPQDALARSGGLSVGERGGI
CIDNQCRTSDPDVLAIGECALWENKIYGLVAPGYQMARAAAATLAGEAGSFSGADMSTKL
KLLGVDVASFGDAQGRTPGCQSYQWTHGPQQVYKKIVVSADGKNLLGGVLVGDAGDYATL
LQMMLNGMALPKHPESLILPALEGSAPKALGVAALPDGAQICSCHNVSKGDICQAVSGGA
GDMAAIKSRTKAATGCGGCSALVKQVMEYQLAEQGVEVKKDICEHFPWSRQEIYHLVRVN
HIRTFEQLIARYGQGHGCEVCKPLVASVLASCWNEYLLKPAHLPLQDTNDRYFANIQKDG
TYSVVPRMAAGEVTPDGLIAIGQIAKRYQLYSKVTGGQRIDLFGARLEQLPAIWRELAEA
GFETGHAYGKSLRTVKSCVGSTWCRYGVQDSTGLAVTLEHRYKGLRAPHKIKMAVSGCTR
ECAEAQGKDIGVIATEKGWNLYVCGNGGMKPRHADLFASDLDEATLIRSIDRLLMFYIRT
ADRLQRTSTWMDNLEGGVDYLREMILEDSLGIGEELEQEMARVVESYQCEWQTTLNDPQR
LALFRSYVNSDEPDETVQRQTLRGQPQLAPFAAQGEPALPSRPWQAICDLDAIPQQAGIG
ARLGERQIALFRFGDQVYALDNLEPGSEANVLSRGLLGDAGGEPIVISPLYKQRIRLRDG
RQCDGGEQAVRAWPVKVENGKVWVGNQQLLARAEAS