Protein Info for BWI76_RS17345 in Klebsiella michiganensis M5al

Annotation: nitrate/nitrite two-component system sensor histidine kinase NarX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details transmembrane" amino acids 154 to 174 (21 residues), see Phobius details PF13675: PilJ" amino acids 40 to 122 (83 residues), 36.9 bits, see alignment E=8.1e-13 PF00672: HAMP" amino acids 173 to 224 (52 residues), 43.9 bits, see alignment 5.9e-15 PF07730: HisKA_3" amino acids 390 to 453 (64 residues), 65.8 bits, see alignment E=1e-21 PF13581: HATPase_c_2" amino acids 491 to 582 (92 residues), 28.8 bits, see alignment E=2.7e-10 PF02518: HATPase_c" amino acids 496 to 585 (90 residues), 55.5 bits, see alignment E=1.8e-18

Best Hits

Swiss-Prot: 78% identical to NARX_SHIFL: Nitrate/nitrite sensor protein NarX (narX) from Shigella flexneri

KEGG orthology group: K07673, two-component system, NarL family, nitrate/nitrite sensor histidine kinase NarX [EC: 2.7.13.3] (inferred from 90% identity to kva:Kvar_1966)

MetaCyc: 78% identical to sensor histidine kinase NarX (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Nitrate/nitrite sensor protein (EC 2.7.3.-)" in subsystem Nitrate and nitrite ammonification (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B560 at UniProt or InterPro

Protein Sequence (592 amino acids)

>BWI76_RS17345 nitrate/nitrite two-component system sensor histidine kinase NarX (Klebsiella michiganensis M5al)
MLKRLFTPLTLVNQLALIVLLATLIGVAGMAISSRLVHGVQGSAHAINKAGSLRMQSYRL
LAAVPLGENDEKLLDEMTATVFSPELQYAARHDDQQRQLQALQHYWQLELAPGMRKAQNQ
ASVATDVAGFVDRIDQLVTAFDHTTEERIKHVVWIQRIMAVGMALLLVFTIVWLRARLLR
PWKQLLGMARAVSRRDFTQRAQISGRNEMATLGMALNNMSEELAESYSVLERRVQEKTAG
LEQKNEILAFLWQANRRLHSNAPLCERISPVLNGLQGLTLLRDIEVRVYDLEDEDNHQEF
SCHSDIQCDDRGCYLCPRDLPPLPDTGTTLKWRLSDAHNQYGILLATLPTGRHLSHDQQQ
LVDTLVEQLTGTLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
DDLPAASRQLLGQIRNELNTSWAQLRELLTTFRLQLTEPGLRPALEASCQEYSAHFGFAV
RLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASATEVSVSVTLRENQVRLVVADNGRG
VPDHAERSNHYGLIIMRDRAQSLRGDCQVRRRETGGTEVVVTFIPEKSFSIQ