Protein Info for BWI76_RS17255 in Klebsiella michiganensis M5al

Annotation: thymidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00265: TK
  • amino acids 2 to 186 (185 residues), 211.7 bits, E=3.6e-67

Best Hits

Swiss-Prot: 88% identical to KITH_SHIDS: Thymidine kinase (tdk) from Shigella dysenteriae serotype 1 (strain Sd197)

KEGG orthology group: K00857, thymidine kinase [EC: 2.7.1.21] (inferred from 92% identity to kpu:KP1_3312)

MetaCyc: 87% identical to thymidine/deoxyuridine kinase (Escherichia coli K-12 substr. MG1655)
Deoxynucleoside kinase. [EC: 2.7.1.145, 2.7.1.21]; 2.7.1.145,2.7.1.21 [EC: 2.7.1.145, 2.7.1.21]

Predicted SEED Role

"Thymidine kinase (EC 2.7.1.21)" in subsystem Folate Biosynthesis (EC 2.7.1.21)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleosides salvage (4/5 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.145 or 2.7.1.21

Sequence Analysis Tools

See A0A285B5C5 at UniProt or InterPro

Protein Sequence (205 amino acids)

>BWI76_RS17255 thymidine kinase (Klebsiella michiganensis M5al)
MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTLVYTAEIDDRFGSGKVSSRIGLSSPAR
LYNTQTALFADIAAEHKRQPIHCVLVDESQFLTREQVRDLSEVVDQLDIPVLCYGLRTDF
RGELFTGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQEGRPYNEGEQVVIGGNERYVS
VCRKHYKEALSEGSLTAVQNKQHIC