Protein Info for BWI76_RS17175 in Klebsiella michiganensis M5al
Annotation: 6-phosphofructokinase II
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to PFKB_ECOLI: ATP-dependent 6-phosphofructokinase isozyme 2 (pfkB) from Escherichia coli (strain K12)
KEGG orthology group: K00850, 6-phosphofructokinase [EC: 2.7.1.11] (inferred from 91% identity to kpn:KPN_02183)MetaCyc: 80% identical to 6-phosphofructokinase 2 (Escherichia coli K-12 substr. MG1655)
Tagatose-6-phosphate kinase. [EC: 2.7.1.144]; 6-phosphofructokinase. [EC: 2.7.1.144, 2.7.1.11]
Predicted SEED Role
"6-phosphofructokinase class II (EC 2.7.1.11)" in subsystem Glycolysis and Gluconeogenesis (EC 2.7.1.11)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
- superpathway of N-acetylneuraminate degradation (22/22 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
- superpathway of hexitol degradation (bacteria) (18/18 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
- superpathway of anaerobic sucrose degradation (18/19 steps found)
- glycolysis I (from glucose 6-phosphate) (13/13 steps found)
- homolactic fermentation (12/12 steps found)
- glycolysis II (from fructose 6-phosphate) (11/11 steps found)
- glycolysis III (from glucose) (11/11 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- galactitol degradation (5/5 steps found)
- glycolysis IV (8/10 steps found)
- 1,3-propanediol biosynthesis (engineered) (7/9 steps found)
- formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
- D-galactosamine and N-acetyl-D-galactosamine degradation (3/4 steps found)
- N-acetyl-D-galactosamine degradation (3/5 steps found)
- lactose degradation I (2/5 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Pentose phosphate pathway
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.11, 2.7.1.144
Use Curated BLAST to search for 2.7.1.11 or 2.7.1.144
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B5B4 at UniProt or InterPro
Protein Sequence (310 amino acids)
>BWI76_RS17175 6-phosphofructokinase II (Klebsiella michiganensis M5al) MTKIYTLTLAPSLDSATQTPQIYPEGKLRCSAPVFEPGGGGINVARAITFLGGSATAIFP VGGATGEHLTALLTDEHVPVDTVETHDWTRQNLHVHVNASGEQYRFVMPGAALTADELRL IEEKVLTIAPGSLLVISGSLPPGISVENLTQLVKNAQRHGLRCIVDSSGDALAAALDVGN IELVKPNQKELSALVQRDLSQPDDVRLAAQELIRSGKVQRVVVSLGAQGALGVDADGSVQ VVPPPMKSQSTVGAGDSMVGAMTLRLAENASLEDMVRFGVAAGSAATINQGTRLCSQENT QKIYDYLRGL