Protein Info for BWI76_RS17065 in Klebsiella michiganensis M5al

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 9 to 792 (784 residues), 1268.3 bits, E=0
• PF01326: PPDK_N
  • amino acids 22 to 349 (328 residues), 431 bits, E=4.9e-133
• PF00391: PEP-utilizers
  • amino acids 386 to 457 (72 residues), 100.7 bits, E=4e-33
• PF02896: PEP-utilizers_C
  • amino acids 483 to 784 (302 residues), 196.6 bits, E=8.1e-62

Best Hits

Swiss-Prot: 94% identical to PPSA_ECOLI: Phosphoenolpyruvate synthase (ppsA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_17080)

MetaCyc: 94% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of N-acetylneuraminate degradation (22/22 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
• superpathway of hexitol degradation (bacteria) (18/18 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
• superpathway of anaerobic sucrose degradation (18/19 steps found)
• gluconeogenesis I (13/13 steps found)
• glycolysis I (from glucose 6-phosphate) (13/13 steps found)
• glycolysis II (from fructose 6-phosphate) (11/11 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (23/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See A0A285B590 at UniProt or InterPro

Protein Sequence (792 amino acids)

>BWI76_RS17065 phosphoenolpyruvate synthase (Klebsiella michiganensis M5al)
MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL
DQSGVNQRIYELLDETDIDDVSALAKAGAQIRQWIIDTPFQSELENAIRDAYTQLSADDA
EASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG
YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE
FYVHKPTLEAGRPAIVRRTMGSKKIRMVYAPTQEHGKQVRIEDVPQAQRDVFSLTDAEVQ
ELAQQALQIEKHYNRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHAQGQIIS
EGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKAAAIVTNRGGRTC
HAAIIARELGIPAVVGCGNATDRIQENQNVTVSCAEGDTGYVYAEILDFSVKSSSVGTMP
DLPLKIMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPGLQNE
IRELMKGYDSPREFYVGRLTEGISTLGAAFFPKRVIVRLSDFKSNEYANLVGGERYEPEE
ENPMLGFRGAGRYVSESFRDCFALECEAVKRVRNDMGLTNVEIMVPFVRTVAQAKAVVEE
LERQGLKRGENGLKLIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVS
ELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTV
VQTWLGLAELNK