Protein Info for BWI76_RS16920 in Klebsiella michiganensis M5al
Annotation: 3-(2,3-dihydroxyphenyl)propionate dioxygenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to MHPB_KLEP3: 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (mhpB) from Klebsiella pneumoniae (strain 342)
KEGG orthology group: K05713, 2,3-dihydroxyphenylpropionate 1,2-dioxygenase [EC: 1.13.11.16] (inferred from 89% identity to kva:Kvar_2154)MetaCyc: 85% identical to 3-carboxyethylcatechol 2,3-dioxygenase (Escherichia coli K-12 substr. MG1655)
3-carboxyethylcatechol 2,3-dioxygenase. [EC: 1.13.11.16]; 1.13.11.16 [EC: 1.13.11.16]
Predicted SEED Role
"2,3-dihydroxyphenylpropionate 1,2-dioxygenase (EC 1.13.11.-)" in subsystem Cinnamic Acid Degradation (EC 1.13.11.-)
MetaCyc Pathways
- 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (6/8 steps found)
- 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (3/5 steps found)
- cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (3/5 steps found)
KEGG Metabolic Maps
- 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
- 2,4-Dichlorobenzoate degradation
- Arachidonic acid metabolism
- Benzoate degradation via hydroxylation
- Biphenyl degradation
- Fluorene degradation
- Naphthalene and anthracene degradation
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.13.11.- or 1.13.11.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B559 at UniProt or InterPro
Protein Sequence (314 amino acids)
>BWI76_RS16920 3-(2,3-dihydroxyphenyl)propionate dioxygenase (Klebsiella michiganensis M5al) MNAYLHCLSHTPLVGYVDPEQAVLDEVNGTIADARARIAAFDPELVVLFAPDHYNGFFYD VMPPFCLGIGATSIGDFSSASGVLPVPTALAEACAHAVIKDGIDLAVSYCMQVDHGFAQP LEFLLGGLDRLPVLPVFINGVASPLPGFQRTRMLGEAIGRFLTTLNKRVLILGSGGLSHQ PPVPELAKADAPMRDRLLGSGRQLPPDERELRQQRVISAAKRFIEDQHSLYPLNPVWDNR FMSLLEQGRLTELDAISNEELSAMAGKSTHEIKTWVAAFAALSAFGRWRCEGRYYRPIPE WIAGFGSLSAAAQN