Protein Info for BWI76_RS16780 in Klebsiella michiganensis M5al

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04879: Molybdop_Fe4S4
  • amino acids 1 to 53 (53 residues), 49.7 bits, 4.3e-17
• TIGR01591: formate dehydrogenase, alpha subunit
  • amino acids 7 to 686 (680 residues), 901.9 bits, E=1.1e-275
• PF00384: Molybdopterin
  • amino acids 61 to 488 (428 residues), 269.2 bits, E=8e-84
• PF01568: Molydop_binding
  • amino acids 576 to 683 (108 residues), 102.6 bits, E=1.9e-33

Best Hits

Swiss-Prot: 66% identical to FDHF_ECOLI: Formate dehydrogenase H (fdhF) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 92% identity to kpu:KP1_3178)

MetaCyc: 66% identical to formate dehydrogenase H (Escherichia coli K-12 substr. MG1655)
RXN0-3281 [EC: 1.17.98.4]; FHLMULTI-RXN [EC: 1.17.98.4]

Predicted SEED Role

"Formate dehydrogenase H (EC 1.2.1.2)" in subsystem Formate dehydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• mixed acid fermentation (16/16 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• hydrogen production V (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• oxalate degradation VI (1/4 steps found)
• methanol oxidation to carbon dioxide (2/6 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.98.4 or 1.2.1.2

Sequence Analysis Tools

See A0A285B529 at UniProt or InterPro

Protein Sequence (716 amino acids)

>BWI76_RS16780 formate dehydrogenase subunit alpha (Klebsiella michiganensis M5al)
MKKITSVCPYCGAGCKLKLVVENNKIIRAEAADGVTNQNQLCLKGYYGWDFLNDTQLLTP
RLQQPMIRYQKGGKFTPVSWDEAIRYTAKKLREIKEKHGPRAIMTTGSSRGTGNETNYVM
QKFARGVLNTNNVDCCARVCHGPSVAGLQLALGNGAMSNSISDIENSKCLLVFGYNCADS
HPIVARRVIKARENGAKIIVCDPRRIETARIADRHLQLNNGSNMALVNAFAHVLLEEDLY
NHDYVDRFTEGLDAYREMVKDYSPEAVEGIVGVPAAEIRQAMRMFAAAPSATIMWGMGVT
QFGQAVDVVRGLASLALLTGNLGRANVGVGPVRGQNNVQGACDMGVLPNMFPGYQDVTDA
GVRAKFANAWGIDPASMDDQVGTRITEVPHKALTGEIKAYYIMGEDPLQTEADLGLVRKG
IEALDFVVVQDIFMTKTAEIADVLLPATSWGEHGGVFTCADRGFQRFEQAIIATGNVKRD
WEIISLLASEMGYPMHYDNNQQIWDEMRELCPLFYGVTYEKMGDMNHVQWPCPTLDHPGT
PWLYKDNLFDTPTGKGQLFAAPWRAPAETPDEAYPLVLCTVREVGHYSCRSMTGNCAALQ
SLADEPGRVQINRADAQRLGIADQQLVWVSSRRGKVITRADISDRINEGAVYMTYQWWVG
ACNELTQDNLDPISKTPETKYCAVKVEAIADQRWAEQYAWTSYSEMKARLKAAVNV