Protein Info for BWI76_RS16755 in Klebsiella michiganensis M5al
Annotation: acetoin reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to BUDC_KLEPN: Diacetyl reductase [(S)-acetoin forming] (budC) from Klebsiella pneumoniae
KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 92% identity to kva:Kvar_2212)MetaCyc: 93% identical to acetoin reductase subunit (Klebsiella pneumoniae)
RXN-11032 [EC: 1.1.1.304]; 1.1.1.- [EC: 1.1.1.304]
Predicted SEED Role
"2,3-butanediol dehydrogenase, S-alcohol forming, (R)-acetoin-specific (EC 1.1.1.4) / Acetoin (diacetyl) reductase (EC 1.1.1.5)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.4, EC 1.1.1.5)
MetaCyc Pathways
- superpathway of (R,R)-butanediol biosynthesis (5/5 steps found)
- pyruvate fermentation to (R)-acetoin I (3/3 steps found)
- pyruvate fermentation to (S)-acetoin (3/3 steps found)
- superpathway of 2,3-butanediol biosynthesis (5/6 steps found)
- (R,R)-butanediol biosynthesis (1/1 steps found)
- (R,R)-butanediol degradation (1/1 steps found)
- meso-butanediol biosynthesis I (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.303, 1.1.1.4
Use Curated BLAST to search for 1.1.1.303 or 1.1.1.304 or 1.1.1.4 or 1.1.1.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B4Z1 at UniProt or InterPro
Protein Sequence (256 amino acids)
>BWI76_RS16755 acetoin reductase (Klebsiella michiganensis M5al) MKKVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATAQAVADEINRSGGRALAVKVDV SQRDQVFAAVEQARKGLGGFDVIVNNAGVAPSTPIEEIREDVIDKVYNINVKGVIWGIQA AVEAFKQEGHGGKIINACSQAGHVGNPELAVYSSSKFAVRGLTQTAARDLAHLGITVNGY CPGIVKTPMWAEIDRQVSEAAGKPLGYGTQEFAKRITLGRLSEPEDVAACVSYLAGPDSN YMTGQSLLIDGGMVFN