Protein Info for BWI76_RS16405 in Klebsiella michiganensis M5al

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00234: tyrosine--tRNA ligase" amino acids 2 to 424 (423 residues), 488.9 bits, see alignment E=6.5e-151 PF00579: tRNA-synt_1b" amino acids 31 to 328 (298 residues), 308.9 bits, see alignment E=3.8e-96 PF01479: S4" amino acids 359 to 403 (45 residues), 26.1 bits, see alignment 5.6e-10

Best Hits

Swiss-Prot: 98% identical to SYY_KLEP7: Tyrosine--tRNA ligase (tyrS) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 97% identity to cro:ROD_14301)

MetaCyc: 94% identical to tyrosine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tyrosine--tRNA ligase. [EC: 6.1.1.1]; 6.1.1.1 [EC: 6.1.1.1]

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4L9 at UniProt or InterPro

Protein Sequence (424 amino acids)

>BWI76_RS16405 tyrosine--tRNA ligase (Klebsiella michiganensis M5al)
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEDTVQDWVDKIRKQVAPFLDFDCGDNS
AIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDQGISFTEFSYNLLQG
YDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDMAEINALEEEDKNSGKAPRAQ
YVLAEQVTRLVHGEEGLVAAKRITESLFNGNLSDLSEADFEQLAQDGVPMIEMEKGADLM
QALVDSELQPSRGQARKTIASNAVTINGEKQSDPEYVFVDGDRLFGRYTLLRRGKKNYCL
VCWK