Protein Info for BWI76_RS16165 in Klebsiella michiganensis M5al

Annotation: peptidase M20

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 TIGR01891: amidohydrolase" amino acids 15 to 418 (404 residues), 408.3 bits, see alignment E=1.6e-126 PF01546: Peptidase_M20" amino acids 112 to 428 (317 residues), 130.9 bits, see alignment E=6.1e-42 PF07687: M20_dimer" amino acids 231 to 319 (89 residues), 40.6 bits, see alignment E=2.2e-14

Best Hits

Swiss-Prot: 82% identical to IAAH_ENTAG: Indole-3-acetyl-aspartic acid hydrolase (iaaH) from Enterobacter agglomerans

KEGG orthology group: K12940, aminobenzoyl-glutamate utilization protein A (inferred from 90% identity to kpn:KPN_01534)

MetaCyc: 74% identical to p-aminobenzoyl-glutamate hydrolase subunit A (Escherichia coli K-12 substr. MG1655)
3.5.1.-

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4R6 at UniProt or InterPro

Protein Sequence (436 amino acids)

>BWI76_RS16165 peptidase M20 (Klebsiella michiganensis M5al)
MPQLYDFINQLAPKMTEWRRDFHLHAESGWLEFRTASKVAEILDDLGYQLALGRDVIDAD
SRMGLPDEETLAQAFQRARAQGAPERWLPAFEGGFAGVVATLDTGRAGPTLAFRVDMDAL
DLNEQHDDSHRPHRDRFASCNEGMMHACGHDGHTAIGLGLAHVLKEYAVQLNGTIKLIFQ
PAEEGTRGARAMVAAGVLDDVDYFTAIHIGTGVPAGTVVCGGDNFMATTKFDVQFSGVAA
HAGGKPEDGRNALLAAAQAALALHAIAPHSAGVSRVNVGVMQAGTGRNVVPSSALLKVET
RGETDAINQYVFERAKQAIAGAATMYEASYQLHLMGAATSSAPSPAWVDYLRQQTAQVPG
VKQAVDRIAAPAGSEDATLMMARVQERGGLASYMIFGTELSAGHHNEKFDFDESVMTQAV
ATLARIALNFPWQRGV