Protein Info for BWI76_RS16100 in Klebsiella michiganensis M5al

Annotation: flavocytochrome c

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 925 PF00724: Oxidored_FMN" amino acids 8 to 341 (334 residues), 240.8 bits, see alignment E=1.3e-74 PF04205: FMN_bind" amino acids 425 to 461 (37 residues), 22.8 bits, see alignment (E = 6.1e-08) PF03486: HI0933_like" amino acids 482 to 672 (191 residues), 38.2 bits, see alignment E=3.8e-13 PF01266: DAO" amino acids 483 to 599 (117 residues), 33.3 bits, see alignment E=2e-11 PF00890: FAD_binding_2" amino acids 483 to 905 (423 residues), 272.8 bits, see alignment E=3.2e-84 TIGR01813: flavocytochrome c" amino acids 483 to 916 (434 residues), 489.4 bits, see alignment E=4.8e-151 PF12831: FAD_oxidored" amino acids 483 to 668 (186 residues), 57.4 bits, see alignment E=8.6e-19 PF13450: NAD_binding_8" amino acids 486 to 528 (43 residues), 28.7 bits, see alignment 6.4e-10

Best Hits

KEGG orthology group: K00244, fumarate reductase flavoprotein subunit [EC: 1.3.99.1] (inferred from 94% identity to kpu:KP1_2565)

MetaCyc: 94% identical to fumarate reductase (NADH) (Klebsiella pneumoniae)
Fumarate reductase (NADH). [EC: 1.3.1.6]

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.1.6 or 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4F8 at UniProt or InterPro

Protein Sequence (925 amino acids)

>BWI76_RS16100 flavocytochrome c (Klebsiella michiganensis M5al)
MTSNQRILHPFTLPNGTELKNRLLMAPMTTCTGYFDGTVTSELVEYYRARAGSIGAIIVE
CCFIDDYGLAFPGAIGIDNDEKVAGLAKIAAAIKAEGSKAILQIYHGGRMVDPQLIGGRQ
PVAPSAIAAPRDGAATPRALSGEEVEGMIAKFGEGVRRAIQAGFDGVEIHGANTYLIQQF
YSPNSNQRDDEWGGSRDNRARFPLAVLDITHKMARQYADDAFIIGYRFSPEEMEVPGIRF
ADTMYLLEKLAARGVGYLHFSVGATLRPSIVDTSDPTPLIEKYCAMRSETLAQVPVMGVG
GVVNAADAGQGLDHGYDLMAVGRACIAYPDWTARIAAGEELELFIDSTQREALNIPEPLW
RFSLVEAMIRDMSMGDAKFKPGMFVETVQDDANELVINVSLENDRIADIALAASPVQTVE
FTTSFEEIRERILSANTPHVDAISGATSQSEAVKKAVSKAMLKSSKALAAEEGAGDAAPK
SYDVVVVGSGGAGLAAAIQAHDEGASVLIVEKMPTIGGNTIKASAGMNAAETRFQRVKGI
EDSKELFYQETLKGGKNKNDPQLLRRFVENAPEAIEWLARRGIMLNDITTTGGMSIDRTH
RPRDGSAVGGYLISGLVRNITKRGIDVLLDTSVEEILMSGDEVSGVRLLTDENESVVVQT
KSIVVATGGFSANSAMVVKYRPDLAGFVTTNHKGATGGGIALLERIGAGTVDMGEIQIHP
TVEQQTSYLISESIRGGGAILVNPQGNRFFNEMETRDKVSAAIIALPEHFAYIVFDEHVR
AKNKAADEYIAKGFVTSASSPRQLAEKLGMDYHAFLNTLERYNGFVEKQHDDDFGRTTAL
RAPINEGPYHAIRIAPGVHHTMGGVTVNTDTAVLNAGHQVIAGAFAAGEVVGGIHGGNRI
GGNAVADIIIFGTLAGHQAAKRARS