Protein Info for BWI76_RS16065 in Klebsiella michiganensis M5al

Annotation: malate:quinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 31 to 517 (487 residues), 762.3 bits, E=1e-233
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 32 to 514 (483 residues), 674.1 bits, E=5.9e-207

Best Hits

Swiss-Prot: 81% identical to MQO_CROS8: Probable malate:quinone oxidoreductase (mqo) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: None (inferred from 93% identity to eae:EAE_18245)

MetaCyc: 68% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Sequence Analysis Tools

See A0A285B4E5 at UniProt or InterPro

Protein Sequence (551 amino acids)

>BWI76_RS16065 malate:quinone oxidoreductase (Klebsiella michiganensis M5al)
MPAIKNTIISMTALAMFVSTATFANDDTSKKTDFLLIGGGIMSASLGTWLHELQPDWKQV
MVEKLDGVALESSNGWNNAGTGHSANMELNYTPERPDGTIDVSKALDINEQFMISRQFWT
AQVKRGILHDPHSFINSTPHMSFVWGDNVDYLQKRYAALQQTTLFQGMKFSTDHAQIKEW
APLVMEGRDPQQRVAATWTPVGTDVNYGEITRQLIGSLKKNNNFTLQTSSEVTDFKRNAD
NSWHVTIKNVNSGEEQAIDAKYVFIGAGGGALKLLQKTGIPEADNYAGFPVGGSFLMTEN
PAVTKAHQQKVYGQASVGAPPMSVPHLDARFLDGKRVVLFGPFATFSTKFLKNGSFLDLL
STTTTDNVLPMTHVGLDNFDLVKYLISQVMLSDDDRFAALKEYYPSARKEDWKLIQAGQR
VQIIKKDAEKGGVLKLGTEVVVDEQKTISALLGASPGASTAAPITLNVIKQMFPEQFNSA
EWQRRIHDIVPSYGQKLNGNVALTQQVWDDTAAALQLTKPPVIEMNATAPVMTAKPAEPK
AETSPQHDMAL