Protein Info for BWI76_RS16020 in Klebsiella michiganensis M5al

Annotation: malonate decarboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 551 TIGR01110: malonate decarboxylase, alpha subunit" amino acids 11 to 551 (541 residues), 1067.8 bits, see alignment E=0 PF16957: Mal_decarbox_Al" amino acids 11 to 551 (541 residues), 986.8 bits, see alignment E=1.2e-301

Best Hits

Swiss-Prot: 58% identical to MADA_MALRU: Acetyl-S-ACP:malonate ACP transferase (madA) from Malonomonas rubra

KEGG orthology group: K13929, malonate decarboxylase alpha subunit [EC: 2.3.1.187] (inferred from 98% identity to kpe:KPK_2894)

MetaCyc: 98% identical to malonate decarboxylase acetyl-[acp]:malonate-[acp] transferase component (Klebsiella pneumoniae)
Biotin-independent malonate decarboxylase. [EC: 4.1.1.88]; Acetyl-S-ACP:malonate ACP transferase. [EC: 4.1.1.88, 2.3.1.187]

Predicted SEED Role

"Malonate decarboxylase alpha subunit" in subsystem Malonate decarboxylase

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.187, 4.1.1.88

Use Curated BLAST to search for 2.3.1.187 or 4.1.1.88

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4N3 at UniProt or InterPro

Protein Sequence (551 amino acids)

>BWI76_RS16020 malonate decarboxylase subunit alpha (Klebsiella michiganensis M5al)
MNAEQTTGRVWNRRRTEKQRRLAEAKISGKVIPTDQLVAVLEHLLAPGDRVVLEGNNQKQ
ADFLSRMLAEVNPQKIHDLHMIMPSVGRSEHLDLFEKGIARKLDFSFSGTQSLRISQLLE
DGLLEIGAIHTYIELYSRLYVDLSPNVALIAGYKADRKGNLYTGPSTEDTPALVEAAAFH
DGIVIAQVNELVDDECDLPRVDIPGSWIDYIVVADKPFFIEPLFTRDPRLIKQEHILMAM
MAIKGIYAEHQVQSLNHGIGFNTAAIELLLPTYGEQLGLKGKICKHWTLNPHPTLIPAIE
SGWVESVHCFGGELGMEEYIRARPDIFFTGADGSMRSNRAFCQLAGQYAVDMFIGSTLQV
DGYANSSTVTRGRLSGFGGAPNMGHDPHGRRHATPAWLNMITEPDPMQRGKKLVVQMVET
FQAGVKPTFVEKLDAVEVAKTSGMPLAPVMIYGDDVTHVLTEEGIAYLYRAESLEERRAM
VAAVAGITDIGLGVDAKRVAELRQSGKVVYPEDIGIRRSDATRSLLAAGSVADLVEWSDG
LYNPPAKFRSW