Protein Info for BWI76_RS15420 in Klebsiella michiganensis M5al

Annotation: beta-D-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1035 PF02837: Glyco_hydro_2_N" amino acids 59 to 225 (167 residues), 208.6 bits, see alignment E=1.6e-65 PF00703: Glyco_hydro_2" amino acids 228 to 341 (114 residues), 52.5 bits, see alignment E=1.9e-17 PF02836: Glyco_hydro_2_C" amino acids 343 to 636 (294 residues), 384.9 bits, see alignment E=6.5e-119 PF16353: LacZ_4" amino acids 649 to 734 (86 residues), 69.8 bits, see alignment E=5e-23 PF02929: Bgal_small_N" amino acids 765 to 1032 (268 residues), 194.1 bits, see alignment E=8.4e-61

Best Hits

Swiss-Prot: 79% identical to BGAL1_KLEP7: Beta-galactosidase 1 (lacZ1) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 78% identity to eae:EAE_18475)

MetaCyc: 60% identical to beta-galactosidase (Escherichia coli K-12 substr. MG1655)
5.4.1.-; Lactase. [EC: 3.2.1.108]; 3.2.1.- [EC: 3.2.1.108]; 3.2.1.- [EC: 3.2.1.108]

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.108 or 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4K1 at UniProt or InterPro

Protein Sequence (1035 amino acids)

>BWI76_RS15420 beta-D-galactosidase (Klebsiella michiganensis M5al)
MQQHDSISAAGATFHQVLVREDWQNQTITHLNRLPAHPTFASWRDPDAARENRPSASRRR
LDGQWQFSLARSPFAVDARWLEDDLPDSRSTPVPSNWQMEGYDAPIYTNVRYPIDTTPPR
VPEENPTGCYSLTFSVDEDWQANGQTQIIFDGVNSAFHLWCNGEWVGYSQDSRLPAAFDL
SPFLQPGDNRICVMVMRWSAGTWLEDQDMWRMSGIFRSVWLLNKPTLHLSDVQLTPQLDA
LYRDAELLVNVSVAAPVAQLEELTVKVELWDEDRLVASHRQPPGSPIIDERGNYAERAAI
RLPVEKPALWSAETPNCYRAVVSLWRGDETIEAEAWDIGFRRVEIKNGLLLLNGKPLLIR
GVNRHEHHHQRGQVVTEEDMVQDILLMKQNNFNAVRCSHYPNAPRWYELCNRYGLYVVDE
ANIETHGMVPMNRLSDDPAWLPAFSARVSRMLQSNRNHPSIIIWSLGNESGGGGNHEAMY
HWLKRNDPSRPVQYEGGGADSTTTDIICPMYARVERDQLIPTVPKWGIKKWISLPGEQRP
LILCEYAHAMGNSLGNFADYWQAFRDYPRLQGGFIWDWADQAISKTFDDGSVGYAYGGDF
GDKPNDRQFCMNGLVFPDRRPHPSLIEAKHAQQYFQFALLAQSPLRIGVSSEYLFRATDN
EELRWQVQAAGETFAEGQVKLELSPEGQSELTLCEALALPAGAEEIWLTLEVVQPQATAW
SDAGHRVAWQQFPIAAPLALRRPAPTGTAPALDGSDAAWTVRSGAQQWTVDRESGLLTRW
QVDGVEQLLTPLRDQFVRAPLDNDIGVSEVERIDPNAWVERWKSAGLYGLSARCVACDAQ
RLAHEVVIDSRWHYLRGDEVVIVSHWRMTFDSEGKLHLAVDGERAGTLPPLPRIGLTFQV
PDQQQPVSWLGYGPHENYPDRRTSACFSRWQLPLEEMTTPYIFPTENGLRCDSKALDWGR
WHVGGDFHFSVQPYSTAQLMETDHWHRMKPEKGVWIALDAQHMGIGGDDSWTPSVLQQWL
LLERQWQYQLTIHFQ