Protein Info for BWI76_RS15405 in Klebsiella michiganensis M5al

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 PF04940: BLUF" amino acids 2 to 91 (90 residues), 81.7 bits, see alignment E=3.5e-27 PF00563: EAL" amino acids 172 to 388 (217 residues), 176.4 bits, see alignment E=6.7e-56

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4H5 at UniProt or InterPro

Protein Sequence (405 amino acids)

>BWI76_RS15405 diguanylate phosphodiesterase (Klebsiella michiganensis M5al)
MLTTLIYRSQMHLTQETDLILLVEKANAENAARGITGILLLKDNVYLQILEGDECVLEQL
FSKIKQDERHSQVVELMRDYAPRRRFENVGMMFFDLNKLQTADVLRKVRQLSQLKGYLST
EERVYKFIHTFISQKSVAAPSPFLRPDKWSLHSRKHTFHVPQESFFAGQCCQFAFQPIIE
PLAGNITSLEALIRDKEGGSPANFFASIPQEQRYEVDLKAKSVAFALAKEINIGDHKISI
NILPMSLVVIPDAIEYLLQEIKKQGLVPEQVIVEVTEDEMISGFSQFSSAIKKLRVSGMG
LAIDDFGAGYAGLSLLTKIQPDKVKIDREIITDIHQSGPKQAVVRSIINCCRELEIAVVA
EGIERIEEWCWLESAGIKRFQGFLFAMPKVNGVGDIRWPVKKSEV