Protein Info for BWI76_RS14745 in Klebsiella michiganensis M5al

Annotation: NAD-dependent epimerase/dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 351 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF01370: Epimerase" amino acids 10 to 241 (232 residues), 48 bits, see alignment E=1.2e-16 PF22917: PRISE" amino acids 63 to 351 (289 residues), 81.9 bits, see alignment E=5.2e-27

Best Hits

KEGG orthology group: None (inferred from 83% identity to rah:Rahaq_1632)

Predicted SEED Role

"Nucleoside-diphosphate-sugar epimerases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B463 at UniProt or InterPro

Protein Sequence (351 amino acids)

>BWI76_RS14745 NAD-dependent epimerase/dehydratase (Klebsiella michiganensis M5al)
MNKKPQPRIALVAGASGIVGRQLVKTLLRHRWEVIGLSRHASPHPDDIPVINVDLRDARD
SAQKLQSLNGITHIFYSAWVNAANWTEMVEPNVAMLRNLVSNIEKTSPLRTVSLMQGYKV
YGAHLGPFKTPARESDPGVPGAEFNAAQLTWLSQFQRGKTWRWSALRPGVVGSTVPGNAM
NLALSIALYASLCRAQNLPLRFPGSEQTWRSIVDHTDGGLLAEATLWAATSPQAENQAFN
VNNGDLWRWCELWPRIADWFELPSAPPVRLSFHQLFVDYRAQWRELAGQDLVEADILRLN
DGTFADFVFSWNYDMFGDGSKLRRAGFTDMQATDDMFFRLFAQLRAARVIP