Protein Info for BWI76_RS14550 in Klebsiella michiganensis M5al

Annotation: 2,3-dihydroxybenzoate-AMP ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 transmembrane" amino acids 75 to 95 (21 residues), see Phobius details PF00501: AMP-binding" amino acids 20 to 380 (361 residues), 240.6 bits, see alignment E=2.6e-75 PF13193: AMP-binding_C" amino acids 431 to 506 (76 residues), 51.1 bits, see alignment E=2.2e-17

Best Hits

KEGG orthology group: K04783, yersiniabactin salicyl-AMP ligase [EC: 6.3.2.-] (inferred from 88% identity to ypp:YPDSF_1218)

MetaCyc: 88% identical to salicyl-AMP ligase (Yersinia pestis)
RXN-11113 [EC: 6.2.1.61, 6.2.1.65]; 2.3.1.- [EC: 6.2.1.61, 6.2.1.65]

Predicted SEED Role

"2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [bacillibactin] siderophore" (EC 2.7.7.58)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.58, 6.3.2.-

Use Curated BLAST to search for 2.7.7.58 or 6.2.1.61 or 6.2.1.65 or 6.3.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (525 amino acids)

>BWI76_RS14550 2,3-dihydroxybenzoate-AMP ligase (Klebsiella michiganensis M5al)
MHSSFDSLIKQYPFPIAEQLRHWAARYPSRIAVVDAKDSLTYSALDLQVDELAAGLSSLG
LRPGEHVLIQLPNGNAFVILLFALLRLGVIPVLAMPSQRALDIDALMALAQPAAYVIHGE
SHADLARQMARKHACLRQVLVASETEGDDFTPLFSLRGERQTWLQPDAAGTALLLLSGGT
TGTPKLIPRRHADYSYNFSASAELCGLNPQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSGSCDEVMPLIAEQGVTHVALVPALAQLWVQAREWEDSDLSSLRVIQVGGARLDPT
LAEQVISTFDCPLQQVFGMAEGLLCFTRLDDPLATVLHSQGRPMSPLDEIRVVDEEENDV
APGETGQLLTRGPYTITGYYRAPEHNARAFTAQGFYRTGDNVRLDEAGNLYVEGRIKEQI
NRAGEKIAAAEVESVLMRLEDVKDCAVVAAPDTLLGERICAFIIAQRTPSDYQQLRQKLV
QMGISAWKIPDQIEFLTHWPLTAVGKVDKKRLTALAIERYRHSAQ