Protein Info for BWI76_RS14530 in Klebsiella michiganensis M5al

Annotation: non-ribosomal peptide synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 200 400 600 800 1000 1200 1400 1600 1800 2032 transmembrane" amino acids 627 to 648 (22 residues), see Phobius details amino acids 758 to 777 (20 residues), see Phobius details amino acids 1265 to 1282 (18 residues), see Phobius details amino acids 1723 to 1743 (21 residues), see Phobius details PF00550: PP-binding" amino acids 22 to 83 (62 residues), 38.4 bits, see alignment (E = 3e-13) amino acids 1415 to 1474 (60 residues), 36.1 bits, see alignment (E = 1.6e-12) amino acids 1948 to 2010 (63 residues), 32.5 bits, see alignment (E = 2.1e-11) PF00668: Condensation" amino acids 149 to 533 (385 residues), 80.7 bits, see alignment E=2.4e-26 amino acids 1530 to 1905 (376 residues), 88.1 bits, see alignment E=1.4e-28 PF00501: AMP-binding" amino acids 575 to 920 (346 residues), 293.9 bits, see alignment E=4.2e-91 TIGR01733: amino acid adenylation domain" amino acids 594 to 990 (397 residues), 400.7 bits, see alignment E=3.3e-124 PF08242: Methyltransf_12" amino acids 1182 to 1279 (98 residues), 30.3 bits, see alignment 1.5e-10

Best Hits

Swiss-Prot: 87% identical to HMWP2_YERE8: High-molecular-weight protein 2 (irp2) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K04784, yersiniabactin nonribosomal peptide synthetase (inferred from 87% identity to ecz:ECS88_2037)

Predicted SEED Role

"iron aquisition yersiniabactin synthesis enzyme (Irp2)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (2032 amino acids)

>BWI76_RS14530 non-ribosomal peptide synthetase (Klebsiella michiganensis M5al)
MISGAPSQDPLLSDNGEAADYQQLRELLIQELNLAPQQLQEESNLIQAGLDSIRLMRWLH
WFRKKGYRLTLRELYAAPTLAAWRQLMRSRSGEKPDDASSPADAAWPVMSEGTPFPLTPV
QHAYLTGRMPGQTLGGVGCHLYQEFAGHYLTAPQLEQAITILLQRHPMLHIAFRADGQQV
WLPQPYWNGVTVHDLRQTDEASRQAYLETLRQRLSHRLLRVEMGETFDFQLTLLPDNRHR
LHVNIDLLIMDASSFTLFFDELNALLAGESLPPGDPRYDFRAYLLHQQKINQPLLDKARV
YWLAKASMLPPAPVLPLACEPATLREVRNTRRRMIVPTTRWNAFSQRAGENGVTPTMALA
TCFAAVLGRWGGLTRLLLNITLFDRQPLHPAVDEMLADFTNILLLDTACDGDTVSNLARK
NQLTFTEDWEHRHWSGVELLRELKRQQSHPHGAPVVFTSNLGRSLYSSRAESPLGEPEWG
ISQTPQVWIDHLAFEHRGEIWLQWDSNDALFPPALVETLFNAYCQLINQLCDDESAWKKP
FADRMPQSQREIRQRVNATDAPVPQGLLHEGIFRIALRQPQALAVTDAHYQWNYRELTEN
ARRCAGRLIACGVQPGDNVAITMSKGAGQLVAVLAVLLSGAVYVPVSLDQPAARRRKIYA
DANVRLVLTCQHDASAWSDDIPQLTWQQAIEAEPLADQAARAPTQPAYIIYTSGSTGTPK
GVVISHRAALNTCCDINTRYQVGPGDRVLALSALHFDLSVYDIFGVLSAGGALVIVMENQ
RRDPHAWCELIQRHQVTLWNSVPALFDMLLTWCEGFADAAPEKLRAVMLSGDWIGLDLPA
RYHAFRPQGQFIAMGGATEASIWSNACEINRVPDHWRAIPYGFPLANQRYRVVDELGRDC
PDWVPGELWIGGIGVAEGYFNDPVRSEQQFVTQSNARWYRTGDLGCYWPDGTLEFLGRRD
KQVKVGGYRIELGEIESALSQLAGVKQSTVVAIGEKEKNLAAWVVPQGSAFCVTHHRDPA
LPQEWRGLAGTLSCCVCPPEISAGQVADFLQHRLLKLKPGQTPGADPLPLMNALAIQPRW
RAVVESWLAFLVTQQRLRPAAEGYQVCAGEAPENNPPSFSGHDLTLTQILRGARHELSLL
NDARWSPESLAFDHPASALYIEELAKICQQLSRRLQRPVRLLEVGVRTARAAECLLTRLS
ADEIEYVGLEHSQELLLSARQRLTPWSDARLALWSADTLAAHAHSADIIWLNNALHRLLP
EEPGLLAALQQLAVPGALLYVLEFRQLTPSALLSTLLLTDGQPEALLHNSADWGAIFTAA
AFNCQHGDEVEGLQRFLVQCPVSQVRRDPRQLQSALAERLPGWMVPKRIVLLDALPLTAN
GKIDYQTLKRCHTPEAENRTEADLPLGDIEMQVAVIWQPLLSMGAVSRETDFFQHGGDSL
LATRLIGQLHQAGYAARLSDLFNHPRLADFAATLRKTDLPVEQPFVHSPEERYRPFALTD
VQQAYLVGRQPGFALGGVGSHFFVEFEIADLDIHRLEKVWNRLIARHDMLRAVVRDGQQR
VLEQTPPWVIPAHILHNPEEALQVRDRLAHQVLNPGVWPVFDLQVGFIDGMPARLWLCLD
NLLLDGLSMQILLSELEHGYRYPQQLPPPLPVTFRDYLQQPALRTPNPDSLTWWQTQLDD
IPPAPALPLRCLPQDVETPRFARLSGAMDSARWRRLKQRAADAHLTPSAVLLSVWSTVLA
AWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPDESWLQSARLLQQRLSESL
NHRDVSAIRVMRQLARRQNVPAVPMPVVFTSALGFEQDNFLARRNLLKPVWGISQTPQVW
LDHQVYESEGELRFNWDFVAALFPDGQVERQFAQYCALLNRMAEDDSSWQLPLAELVPPL
KVTEQCAHRLRPERAQPRIAADESSVSLICDAFREVVGEPVAPTENFFEAGATSLNLVQL
HVLLQRHEFAALTLLDLFTHPSPVALANYLAGVALEEQTKRVRPVRRRQRRI