Protein Info for BWI76_RS14505 in Klebsiella michiganensis M5al
Annotation: salicylate synthase Irp9
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K04781, salicylate synthetase [EC: 4.1.3.- 5.4.4.2] (inferred from 90% identity to ypm:YP_1659)MetaCyc: 90% identical to salicylate synthase monomer (Yersinia enterocolitica)
Isochorismate synthase. [EC: 5.4.4.2]; RXN-1981 [EC: 5.4.4.2, 4.2.99.21]
Predicted SEED Role
"Anthranilate synthase, aminase component (EC 4.1.3.27)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Tryptophan synthesis (EC 4.1.3.27)
MetaCyc Pathways
- superpathway of chorismate metabolism (54/59 steps found)
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- superpathway of menaquinol-8 biosynthesis I (10/10 steps found)
- superpathway of demethylmenaquinol-8 biosynthesis I (9/9 steps found)
- 2-carboxy-1,4-naphthoquinol biosynthesis (7/7 steps found)
- L-tryptophan biosynthesis (6/6 steps found)
- superpathway of demethylmenaquinol-6 biosynthesis I (8/9 steps found)
- superpathway of demethylmenaquinol-9 biosynthesis (8/9 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- 2,3-dihydroxybenzoate biosynthesis (3/3 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of menaquinol-10 biosynthesis (8/10 steps found)
- superpathway of menaquinol-11 biosynthesis (8/10 steps found)
- superpathway of menaquinol-12 biosynthesis (8/10 steps found)
- superpathway of menaquinol-13 biosynthesis (8/10 steps found)
- superpathway of menaquinol-6 biosynthesis (8/10 steps found)
- superpathway of menaquinol-7 biosynthesis (8/10 steps found)
- superpathway of menaquinol-9 biosynthesis (8/10 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- salicylate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- salicylate biosynthesis II (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- enterobactin biosynthesis (7/11 steps found)
- 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
- acridone alkaloid biosynthesis (1/4 steps found)
- vibriobactin biosynthesis (4/9 steps found)
- bacillibactin biosynthesis (6/12 steps found)
- superpathway of phylloquinol biosynthesis (8/15 steps found)
- superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of siderophore group nonribosomal peptides
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Pyruvate metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.-, 4.1.3.27, 5.4.4.2
Use Curated BLAST to search for 4.1.3.- or 4.1.3.27 or 4.2.99.21 or 5.4.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (436 amino acids)
>BWI76_RS14505 salicylate synthase Irp9 (Klebsiella michiganensis M5al) MKISEFLHLALPEEQWLPMISGVLRQFGDEECYVYERQPCWYIGKGCQAQLQINADGTQA IFIDDAGEQKWAVDSITDCARRFMTHPRVRGCRVYGQVGFNFAAHARGMAFDAGEWPLLT LTAPREELIFEKGNVTVYADSADGCRRLCEWVKEVDTATPCGPMVVDTALDGEAYKQQVA RAVSAIHRGDYVKVIVSRAIPLPARIDMPATLLYGRQANTPTRSFMFRQQGREALGFSPE LVMSVTGNKVVTEPLAGTRDRMGDTAHNQANERELLHDGKEVLEHILSVKEAIAELEAVC QPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAEDKDAWDAFTVLFPSITASGIPKKAALNA IMQIEKTPRELYSGAILLLEDTRFDAALVLRSVFQDSQRCWIQAGAGIIAQSTPERELTE TREKLASIAPYLKVPA