Protein Info for BWI76_RS14255 in Klebsiella michiganensis M5al

Annotation: terminal oxidase subunit II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 transmembrane" amino acids 6 to 35 (30 residues), see Phobius details amino acids 58 to 78 (21 residues), see Phobius details amino acids 81 to 99 (19 residues), see Phobius details amino acids 117 to 140 (24 residues), see Phobius details amino acids 154 to 179 (26 residues), see Phobius details amino acids 191 to 212 (22 residues), see Phobius details amino acids 229 to 247 (19 residues), see Phobius details amino acids 259 to 281 (23 residues), see Phobius details amino acids 300 to 322 (23 residues), see Phobius details TIGR00203: cytochrome d ubiquinol oxidase, subunit II" amino acids 4 to 329 (326 residues), 157.8 bits, see alignment E=2.4e-50 PF02322: Cyt_bd_oxida_II" amino acids 4 to 323 (320 residues), 324 bits, see alignment E=5.4e-101

Best Hits

KEGG orthology group: K00426, cytochrome bd-I oxidase subunit II [EC: 1.10.3.-] (inferred from 75% identity to pao:Pat9b_3271)

MetaCyc: 66% identical to cyanide insensitive ubiquinol oxidase subunit II (Pseudomonas putida KT2440)
RXN-6883 [EC: 1.10.3.11]

Predicted SEED Role

"putative Cytochrome bd2, subunit II" in subsystem Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 1.10.3.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (334 amino acids)

>BWI76_RS14255 terminal oxidase subunit II (Klebsiella michiganensis M5al)
MIDLSLIWFGIIIFSILMYIVMDGFDLGIGIIFPFSQDAKERDVMVNTVAPVWDGNETWL
VMGGAGLYGAFPLAYAVITDALAAPLTLMLIGLIFRGVAFEFRFKATIAHRPFWDKAFIS
GSVIATFSQGVVVGCLINGLPVNHRAYAGSELIWLDPFALFCGLGLVIAYALLGSTWLVM
KTEGQLQLKMAWLTLPLVMALLGVMGIVSLWTPLAHPLIATRWFSLPNLFWFLPVPVLVL
ICSWGVIRSIRRGSHYLPFLLTLMLIFLGFSGLGISIWPYIIPPSVTLYQAASPESSQAF
MLAGSLAIIPIILVYTFWSYYVFRGKVRTGEGYH