Protein Info for BWI76_RS14140 in Klebsiella michiganensis M5al

Annotation: glycogen debranching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 TIGR02100: glycogen debranching enzyme GlgX" amino acids 12 to 689 (678 residues), 1056.5 bits, see alignment E=0 PF02922: CBM_48" amino acids 16 to 104 (89 residues), 71.1 bits, see alignment E=8.2e-24 PF00128: Alpha-amylase" amino acids 189 to 283 (95 residues), 34.6 bits, see alignment E=1.4e-12

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 87% identity to enc:ECL_01951)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3T6 at UniProt or InterPro

Protein Sequence (691 amino acids)

>BWI76_RS14140 glycogen debranching enzyme (Klebsiella michiganensis M5al)
MRKEKAFAIRPGDSQQLGAHFDGKGVNFALFSAHAERVELCLFDPSGKKEIARLELPEYT
HEIWHGYVPDLQPGALYGYRVYGPYDPENGHRFNPHKLLIDPYARELAGDIAWNDAHFGY
ELGHEEKDLSFDTRDSAPFTPKCKVVDPDACDWQGENRPDIRWPNTVIYETHVKGFTQLN
PALPPELRGTFEGLGHQASVEYIKSLGITSVELLPVHWFPDDQHLLDRGLKNFWGYNTLG
FFAPASRYYGPAGIAGFRNMVRAFHDAGIEVILDVVYNHTAEGNELGPTLSFKGIDNFSY
YRTMPDRHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAQSMHVDGFRFDLGTILGREP
EGFDPRGGFFDAITQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYW
KGDNVSTDFAARLLGSGDLYDLRGRRPWASVNFITAHDGFTLNDLVSYNEKHNEANGEDN
NDGHNDNRSCNYGEEGPTENQAIVAVRERQKRNFLTTLFFSHGTPMLLAGDEFGRTQQGN
NNGYCQDSEISWINWEALSEDDHALRHFTQRLIALRAQQPLLRRESWRDGLEIRWFNAGG
GLQQSEQWDEGSTLGLAISRPDLEEEEGIWHDVLMLFNPFEGDVAFQIPQFGEGGWVLEL
STAEVKSDGVTITEAIDFVLAGRSIALFRRP