Protein Info for BWI76_RS14130 in Klebsiella michiganensis M5al

Annotation: malto-oligosyltrehalose trehalohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 595 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 20 to 561 (542 residues), 697.6 bits, see alignment E=4.7e-214 PF00128: Alpha-amylase" amino acids 132 to 210 (79 residues), 46.4 bits, see alignment E=4.1e-16 amino acids 232 to 439 (208 residues), 29.4 bits, see alignment E=6.5e-11 PF11941: DUF3459" amino acids 503 to 586 (84 residues), 50.7 bits, see alignment E=1.9e-17

Best Hits

KEGG orthology group: None (inferred from 76% identity to enc:ECL_01949)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3X3 at UniProt or InterPro

Protein Sequence (595 amino acids)

>BWI76_RS14130 malto-oligosyltrehalose trehalohydrolase (Klebsiella michiganensis M5al)
MESISFHKHWGAEFIAADTVRFRVWAEGQKTMTLSLKTRDIAMEAAGGGWFQIDVSGVKH
GDEYMLLLADGRRIPDPATRAQRDDVNGPSVVIDPRLFQPVNRVWKGRPWEETVIYELHP
GTFTPEGTFLAAIDKLPYLAELGITQVEIMPVAQFGGSRGWGYDGVLLYAPHAAYGSPGD
FHAFIDAAHGLGLSVVLDIVLNHFGPEGNYLSLLSPGFFHQNRMTPWGNAIAYETEAVRQ
YITEAPLFWLTEYHLDGLRFDAIDQIEDRSARHVLIEIAENIRKAIPDRHIHLTTEDSRN
VIFLHPRDEDGQTPLFTAEWNDDFHNAAHVFATGETHAYYQDFAHQPASKLARALAEGFV
YQGELSAQTGKPRGVACAAQPPQFFVDFIQNHDQTGNRAQGERLISLAGAGKTRVLLAAL
LLSPHIPLLFMGEEFGESNPFLFFTDFQGSLAKAVREGRAKEFAGHAGHDKSVPDPNDPL
TFIRSKLDWQKAASEEGKSWLRFTRNLILLRHRYIVPLLAAGGGVEGKILHTEPQALAVS
WQFAGGTLSVALNIGEEPLALPEIPGEVIFSWPEQQEALTPDSIVVRFAHGEASV