Protein Info for BWI76_RS14040 in Klebsiella michiganensis M5al

Annotation: thiaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03070: TENA_THI-4
  • amino acids 17 to 224 (208 residues), 161 bits, E=1.8e-51

Best Hits

KEGG orthology group: K03707, thiaminase (transcriptional activator TenA) [EC: 3.5.99.2] (inferred from 81% identity to kva:Kvar_2503)

Predicted SEED Role

"Thiaminase II (EC 3.5.99.2) involved in salvage of thiamin pyrimidine moiety" (EC 3.5.99.2)

MetaCyc Pathways

• thiamine diphosphate salvage IV (yeast) (5/7 steps found)
• base-degraded thiamine salvage (1/2 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.99.2

Use Curated BLAST to search for 3.5.99.2

Sequence Analysis Tools

See A0A285B3V5 at UniProt or InterPro

Protein Sequence (231 amino acids)

>BWI76_RS14040 thiaminase (Klebsiella michiganensis M5al)
MILPAFTQGIYGRLRQQAGADWQHYVAHPFLRQLADGTLPESAFRRYLTQDYLFLIHFAR
SYALLVSKLRTLAEMRVAAASMNAIIDELPLHVGYCREWGLDEATMAAEAEAVETVNYTR
YVLDIGHSGDALDLLAALMPCVAGYAEIGLGLLHDPATRLTANPYASWIRNYGDEGYLNG
VNNAIGLLETLWQQRGGEARFAELSAIFTTATRLEANFWQMGLNAVAERPA