Protein Info for BWI76_RS14000 in Klebsiella michiganensis M5al

Annotation: glutamine amidotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00117: GATase
  • amino acids 79 to 198 (120 residues), 72.4 bits, E=4.1e-24
• PF07722: Peptidase_C26
  • amino acids 79 to 191 (113 residues), 38.2 bits, E=1.4e-13

Best Hits

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 68% identity to kva:Kvar_2485)

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Sequence Analysis Tools

See A0A285B3Q4 at UniProt or InterPro

Protein Sequence (247 amino acids)

>BWI76_RS14000 glutamine amidotransferase (Klebsiella michiganensis M5al)
MSLSRNTLLLVQTGTPPDEIVSDHGDLPLWFGNLLAPWRNKISVARVYAGEPLPAPDNNT
VAVITGSWDMVTERLPWSEMTAAWIREAMAIEMPLFGVCYGHQLMAHALGGEVDYHPAGR
EAGSKTISLSAHGLTDRLLADHPAPFSAHLTHMQTVTRLPPGATVLASSQHDPHQIVRYG
AHAVSTQFHPEITPAIARSLIAFRQSALRNEGVDPDRLSRDVAESPVASAILTRFVAGYL
QPDLNLA