Protein Info for BWI76_RS13995 in Klebsiella michiganensis M5al

Annotation: penicillin-binding protein 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details TIGR03423: penicillin-binding protein 2" amino acids 20 to 616 (597 residues), 750.2 bits, see alignment E=8.2e-230 PF03717: PBP_dimer" amino acids 65 to 238 (174 residues), 158.1 bits, see alignment E=3.4e-50 PF00905: Transpeptidase" amino acids 273 to 612 (340 residues), 231.8 bits, see alignment E=1.1e-72

Best Hits

Swiss-Prot: 74% identical to MRDA_SALTS: Peptidoglycan D,D-transpeptidase MrdA (mrdA) from Salmonella typhimurium (strain SL1344)

KEGG orthology group: K05515, penicillin-binding protein 2 (inferred from 92% identity to kpe:KPK_2538)

MetaCyc: 59% identical to peptidoglycan DD-transpeptidase MrdA (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3W4 at UniProt or InterPro

Protein Sequence (639 amino acids)

>BWI76_RS13995 penicillin-binding protein 2 (Klebsiella michiganensis M5al)
MSLMRDEIRDHSAEEMLFIRRAVIAFLLVVVCFAILILNLYRLQVDQHDFYQTRSNQNDI
KMLPIAPSRGLIFDRNGIPLVQNITLYRLQVIPSKIADMTALLQQLTPIVDLTPQDIADF
RDDMHHNSRYKEVTLKADLSDVEVARFAVNEFHFPGVTVESYQQRAYPYGAELAHVVGYV
SKINNSDLQKLAKAGEEENYAADHNIGKQGIEGYYEKALHGTTGYQEVEVDNHGRVVRLL
KEVPPVAGKNIYLTLDLHLQQYIESVLKGQRAAVVAVDPRDGGVLAMVSSPSYDPNPFVR
GIGYQAYRSLLDNPDRPLINRVTQGLYPPASTVKPYMALSALSAGVITPTTSFFGAPTWT
LPGTQRRYRDWLKSGHGMLNVTKAIEESADTFFYQVAFEMGIDRIHEWLSKFGYGQSTGI
DLNEEYAGVLPSREWKQRVHKKPWYQGDTISVGIGQGYWIATPIQMVKALTTLINNGKVQ
NPHLLYSMKQGNKVERYQPPQDLPQVGDPRSPYWGVVRNGMYGMANLPNGTGYKLFHTAP
YQIAAKSGTSQVFSLKENQTYNAKMIPVRLRDHIFYTLFAPYQHPKVAMALILENGGGDG
VVAGPTARAILDHIFDPANAPAVQGAASTVPQVESADAQ