Protein Info for BWI76_RS13690 in Klebsiella michiganensis M5al

Annotation: muconate and chloromuconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02534: muconate and chloromuconate cycloisomerases
  • amino acids 5 to 371 (367 residues), 467.3 bits, E=1.8e-144
• PF02746: MR_MLE_N
  • amino acids 14 to 128 (115 residues), 103.1 bits, E=1.2e-33
• PF13378: MR_MLE_C
  • amino acids 152 to 364 (213 residues), 167.8 bits, E=3e-53

Best Hits

Swiss-Prot: 56% identical to CATB_PSEPU: Muconate cycloisomerase 1 (catB) from Pseudomonas putida

KEGG orthology group: K01856, muconate cycloisomerase [EC: 5.5.1.1] (inferred from 90% identity to kva:Kvar_2429)

MetaCyc: 57% identical to muconate cycloisomerase (Pseudomonas reinekei)
Muconate cycloisomerase. [EC: 5.5.1.1]

Predicted SEED Role

"Muconate cycloisomerase (EC 5.5.1.1)" in subsystem Catechol branch of beta-ketoadipate pathway or Muconate lactonizing enzyme family (EC 5.5.1.1)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• superpathway of salicylate degradation (7/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• catechol degradation to β-ketoadipate (4/4 steps found)
• mandelate degradation to acetyl-CoA (14/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (23/35 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation
• Toluene and xylene degradation

Isozymes

Compare fitness of predicted isozymes for: 5.5.1.1

Use Curated BLAST to search for 5.5.1.1

Sequence Analysis Tools

See A0A285B3N6 at UniProt or InterPro

Protein Sequence (372 amino acids)

>BWI76_RS13690 muconate and chloromuconate cycloisomerase (Klebsiella michiganensis M5al)
MTATIERIESWLVDIPTIRPHKLSMTTMGCQTLAIIRVTRSDGICGIGEATTIGGLSYGV
ESPEAIVSAINHYLTPLLKGQAADNLNALTSRMDGAVKGNTFAKSAIETALLDAQGKALG
LPVSALLGGALTTSLPVLWTLASGDTEKDIAEGEKLLAERRHQAFKLKIGARALETDLRH
TRAIVEALGDRASIRVDVNQAWDATVAAKGCRELAAMGVDLIEQPVGAQDNAALVRLSHH
IDTAILADEAVATHYDGYRLAQQGFSGAYALKIAKAGGPNSVLALAKVAQAAGIGLYGGT
MLEGTVGTLASLHAWSTLPLQWGSEMFGPLLLKDDIVSVPLTFAEGEVTLPQTPGLGVEL
DEDKLRFYSRKG