Protein Info for BWI76_RS13670 in Klebsiella michiganensis M5al

Annotation: NarK family nitrate/nitrite MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 transmembrane" amino acids 36 to 60 (25 residues), see Phobius details amino acids 72 to 90 (19 residues), see Phobius details amino acids 101 to 122 (22 residues), see Phobius details amino acids 128 to 150 (23 residues), see Phobius details amino acids 172 to 195 (24 residues), see Phobius details amino acids 209 to 232 (24 residues), see Phobius details amino acids 254 to 277 (24 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 318 to 338 (21 residues), see Phobius details amino acids 346 to 374 (29 residues), see Phobius details amino acids 397 to 418 (22 residues), see Phobius details amino acids 434 to 454 (21 residues), see Phobius details TIGR00886: nitrite transporter" amino acids 34 to 415 (382 residues), 344.7 bits, see alignment E=3.3e-107 PF07690: MFS_1" amino acids 42 to 373 (332 residues), 50.4 bits, see alignment E=8.5e-18

Best Hits

Swiss-Prot: 83% identical to NARU_SALTY: Nitrate/nitrite transporter NarU (narU) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 90% identity to kpe:KPK_2477)

MetaCyc: 82% identical to nitrate/nitrite transporter NarU (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-239

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3F6 at UniProt or InterPro

Protein Sequence (462 amino acids)

>BWI76_RS13670 NarK family nitrate/nitrite MFS transporter (Klebsiella michiganensis M5al)
MQSKNEKKNHYLLKEWKPENAAFWENKGKKIARRNLCISVACLLLAFCVWMLFSAVAVNL
NKVGFNFTTDQLFLLTALPSLSGAILRVPYSFMVPIFGGRYWTVLSTIILVAPCIWLGIA
IQNTATPFWVFITIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQ
MIAPAAIFVPIFAFLGVHGVPQEDGSILALSNAALVWVPLLVIATIAAWVGMNDIASSKA
SIRDQLPVLKRPHMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILKLAFFGPFIGA
LARSFGGIISDRLGGVRVTLVNFVLMALFTGLLFLTLPGTGSGSFLAFYVVFMGLFLTAG
LGSGSTFQMIAVIFRQITIDSVKKRGGSDEQAQHEAVTDTAAALGFISAIGAIGGFFIPK
AFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKPAKK