Protein Info for BWI76_RS13530 in Klebsiella michiganensis M5al

Annotation: amidohydrolase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 7 to 356 (350 residues), 367.2 bits, E=4.7e-114
• PF01546: Peptidase_M20
  • amino acids 63 to 367 (305 residues), 131.2 bits, E=5.2e-42
• PF07687: M20_dimer
  • amino acids 176 to 266 (91 residues), 45.4 bits, E=6.8e-16

Best Hits

Swiss-Prot: 48% identical to YXEP_BACSU: Uncharacterized hydrolase YxeP (yxeP) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 90% identity to kpn:KPN_01902)

MetaCyc: 48% identical to N-acetyl-sulfur-metabolite deacetylase (Bacillus subtilis subtilis 168)
3.5.1.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase homolog (EC 3.5.1.18)" (EC 3.5.1.18)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• S-(2-succinyl)-L-cysteine degradation (3/4 steps found)
• S-benzyl-L-cysteine degradation (2/5 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.18

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

See A0A285B3M4 at UniProt or InterPro

Protein Sequence (373 amino acids)

>BWI76_RS13530 amidohydrolase family protein (Klebsiella michiganensis M5al)
MSLAEQLIRWRRELHQYPELSLQEVDTTARIRDWLQSGGISLLPYDLKTGAVAEVGSGNK
VIALRADIDALPIEEAASVPFRSLNAGVMHACGHDIHTSVILGAALLLKQREAQLAGRVR
ILFQPAEESFGGAKTLIRAGVLEGVSAIFGMHNEPGLPVGEFATRGGAFYANVDRFVFKV
TGKGAHAARPHEGKDAILLASQLVTLLQSVASREVNTLDSVVLSVTRIQGGNTWNVLPES
VELEGTLRTHSSEVQQRVKARVSEIAAGFASAFGAQIDVFWYAGPTALVNDERWAAFASD
VADKSGYRTHHADLHLGGEDFAVYLQHIPGAFVSIGSASEFGLHHPAFNPDERLIAPAAH
YFADLAEQALKEL