Protein Info for BWI76_RS13305 in Klebsiella michiganensis M5al

Annotation: hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 207 PF00857: Isochorismatase" amino acids 14 to 165 (152 residues), 80.5 bits, see alignment E=8.7e-27

Best Hits

Swiss-Prot: 90% identical to YCAC_ECOLI: Probable hydrolase YcaC (ycaC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to esc:Entcl_2642)

Predicted SEED Role

"Nicotinamidase family protein YcaC" in subsystem NAD and NADP cofactor biosynthesis global

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3G7 at UniProt or InterPro

Protein Sequence (207 amino acids)

>BWI76_RS13305 hydrolase (Klebsiella michiganensis M5al)
MAKPYVRLDKNDAAVLLVDHQTGLLSLVRDIDPDKFKNNVLALGDLAKYFALPTILTTSF
ENGPNGPLVPELKAQFPDAPYIARPGNINAWDNDDFVKAVKATGKKQLIIAGVVTEVCVA
FPALSAIEEGFDVFVVTDASGTFNPITRDAAWDRMSQAGVQLMSWFGVACELHRDWRNDV
EGLGTLFSNHIPDYRNLMTSFNLLQQK