Protein Info for BWI76_RS13270 in Klebsiella michiganensis M5al

Annotation: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 790 transmembrane" amino acids 14 to 36 (23 residues), see Phobius details amino acids 42 to 60 (19 residues), see Phobius details amino acids 67 to 87 (21 residues), see Phobius details amino acids 89 to 90 (2 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 123 to 142 (20 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 17 to 787 (771 residues), 1074.9 bits, see alignment E=0 PF13360: PQQ_2" amino acids 196 to 308 (113 residues), 22.7 bits, see alignment E=7.3e-09 amino acids 362 to 510 (149 residues), 22.4 bits, see alignment E=9e-09 amino acids 664 to 749 (86 residues), 24.8 bits, see alignment E=1.7e-09 PF01011: PQQ" amino acids 224 to 254 (31 residues), 23.2 bits, see alignment (E = 4.3e-09) amino acids 441 to 467 (27 residues), 26.9 bits, see alignment (E = 3e-10) amino acids 658 to 685 (28 residues), 20.7 bits, see alignment (E = 2.7e-08) amino acids 719 to 752 (34 residues), 20.8 bits, see alignment (E = 2.4e-08)

Best Hits

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 93% identity to kpe:KPK_2398)

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.25

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3C7 at UniProt or InterPro

Protein Sequence (790 amino acids)

>BWI76_RS13270 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (Klebsiella michiganensis M5al)
MATDNAPRGFPRILQWLLAGLMLIIGLAIGILGAKLASVGGTFYFALMGLVMVIAAVLIF
RSRRGGIMLYAVAFVASIVWAISDAGWTYWPLFSRLFALGVLAFLCALVWPFLSRQPAKK
GPAFGLAAVIAVALLVSFAWMFKSQPLVSASEAVPVKPVAADEQQKNWAHWGNTTHGDRF
AALDQINKQNVDQLQVAWIAHTGDIPQSNGSGAEDQNTPLQIGDTLYVCTPYSKVLALDV
DSGKEKWRYDSKATAPNWQRCRGLGYYEDSQAQVSPAADAQPAACSRRLFLPTTDARLIA
IDADNGKLCDAFGDHGIVDLSIGMGEIKAGYYQQTSTPLVAGNVVVVGGRVADNFSTGEP
PGVVRAYDVHTGKLAWAWDPGNPALTGEPPAGQTYTRGTPNVWSAMSYDAKLNLIYLPTG
NATPDFFGGERTALDDKYSSSIVAVDATTGQVRWHYQTTHHDLWDFDLPSQPLLYDLPDG
KGGTTPVLVQTSKQGMIFMLNRATGEPVAKVEERPVPAGNVKGERYSPTQPYSVGMPMIG
NETLTESDMWGATPVDLLLCRIQFKEMRHQGVFTPPGEDRSLQYPGSLGGMNWGSVSVDP
NNSLIFVNDMRLGLANYMVPRAKVAKDASGIEMGIVPMEGTPFGAMRERFLSPLGIPCQK
PPFGTLSAVDLKTGKLVWQVPVGTVEDTGPLGIRMHMPIPIGMPTLGASLSTQSGLLFFA
GTQDFYLRAFDTANGKEIWKSRLPVGSQSGPMTYVSPKTGKQYIIINAGGARQSPDRGDY
IIAYALPEQK