Protein Info for BWI76_RS13165 in Klebsiella michiganensis M5al

Annotation: ATP-dependent RNA helicase HrpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 TIGR01967: RNA helicase HrpA" amino acids 14 to 1297 (1284 residues), 2256.7 bits, see alignment E=0 PF00270: DEAD" amino acids 84 to 233 (150 residues), 28.7 bits, see alignment E=3.6e-10 PF00271: Helicase_C" amino acids 282 to 403 (122 residues), 45.3 bits, see alignment E=3.4e-15 PF04408: HA2_N" amino acids 466 to 498 (33 residues), 23.2 bits, see alignment (E = 2e-08) PF21010: HA2_C" amino acids 499 to 557 (59 residues), 41.1 bits, see alignment 7.2e-14 PF07717: OB_NTP_bind" amino acids 622 to 698 (77 residues), 57.9 bits, see alignment 3.3e-19 PF11898: DUF3418" amino acids 713 to 1297 (585 residues), 792.3 bits, see alignment E=1.1e-241

Best Hits

Swiss-Prot: 93% identical to HRPA_ECOLI: ATP-dependent RNA helicase HrpA (hrpA) from Escherichia coli (strain K12)

KEGG orthology group: K03578, ATP-dependent helicase HrpA [EC: 3.6.4.13] (inferred from 70% identity to aat:D11S_1569)

MetaCyc: 93% identical to ATP-dependent 3'->5' RNA helicase HrpA (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase HrpA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B355 at UniProt or InterPro

Protein Sequence (1300 amino acids)

>BWI76_RS13165 ATP-dependent RNA helicase HrpA (Klebsiella michiganensis M5al)
MTEQQKITFSQLVTRLDGLMLRDKSRFARRLHGVKKVKNPDAQQAILQEMAQEIEQAAGR
VLLREAARPAITYPENLPVSQKKQEILDAVRDHQVVIVAGETGSGKTTQLPKICMELGRG
VKGLIGHTQPRRLAARTVANRIAEELQTEPGGCIGYKVRFSDHVSDNTMVKLMTDGILLA
EIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLRELLPRRPDLKIIITSATIDPERFSRH
FNNAPIIEVSGRTYPVEVRYRPIVEDADDTERDQLQAIFDAVDELGRESPGDILIFMSGE
REIRDTADALNKLNLRHTEVLPLYARLSNSEQNRVFQAHSGRRIVLATNVAETSLTVPGI
KYVIDPGTARISRYSFRTKVQRLPIEPVSQASANQRKGRCGRVSEGICIRLYSEDDFLSR
PAFTDPEILRTNLASVILQMTALGLGDIAAFPFVEAPDKRNIQDGVRLLEELGAITTDEQ
QTAYKLTPMGRQLSQLPVDPRLARMVLEAQKHGCVREAMIITSALSIQDPRERPMDKQQA
SDEKHRRFHDKESDFLAFVNLWNYLGEQQKALSSNQFRRQCRVDFLNYLRVREWQDIYTQ
LRQVVKELGLPVKSEPAEYREIHTALLTGLLSHIGMKDADKQEFTGARNARFSIFPGSGL
FKKPPKWTMVAELVETSRLWGRIAARIEPEWVEPVAQHLIKRSYSEPHWERGQGAVMATE
KVTVYGLPIVAARKVNYSQIDPALCRELFIRHALVEGDWQTRHAFFRENLKLRNEIEELE
HKSRRRDILVDDETLFEFYDQRISHDVISARHFDKWWKQASRETPDLLNFEKSMLIKEGA
EQVSKLDYPNFWHQGNLKLRLTYQFEPGADADGVTVHIPLPLLNQVEEAGFEWQIPGLRR
ELIIALIKSLPKPVRRNFVPAPNYAEAFLGRATPLELPLLDSLERELRRMTGVTIDREAW
QWEQVPDYLKITFRVVDDKNKKLHEGRSLQDLKDALKGKVQETLSAVADDGIELSGLHIW
SFGRLPESYEQKRGNYKVKAWPALVDERDSVAIKLFDNPLEQQQEMWRGLRRLLLLNIPS
PIKYLHEKLPNKAKLGLYFNPYGKVLDLIDDCISCGVDKLIDEAGGPVWSEEGFAALHEK
VRAELNETVVEIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKSQMSGLVYRGFVTG
NGFRRLGDTLRYLQAIEKRLEKMAIDPHRDRAQMLKVESLQQRWQQWLNKLPPQRRQDDD
VQEIRWMIEELRVSLFAQQLGTPYPISDKRVLQAMEQIAA