Protein Info for BWI76_RS13110 in Klebsiella michiganensis M5al

Annotation: phenylacetic acid degradation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 356 TIGR02160: phenylacetate-CoA oxygenase/reductase, PaaK subunit" amino acids 4 to 351 (348 residues), 525.5 bits, see alignment E=2.6e-162 PF00970: FAD_binding_6" amino acids 7 to 105 (99 residues), 43.7 bits, see alignment E=4.4e-15 PF00175: NAD_binding_1" amino acids 118 to 225 (108 residues), 66.6 bits, see alignment E=4.4e-22 PF00111: Fer2" amino acids 267 to 342 (76 residues), 66.2 bits, see alignment E=3e-22

Best Hits

Swiss-Prot: 74% identical to PAAE_ECOLI: 1,2-phenylacetyl-CoA epoxidase, subunit E (paaE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 89% identity to eae:EAE_20500)

MetaCyc: 74% identical to phenylacetyl-CoA 1,2-epoxidase, reductase subunit (Escherichia coli K-12 substr. MG1655)
RXN0-2042 [EC: 1.14.13.149]

Predicted SEED Role

"Phenylacetate-CoA oxygenase/reductase, PaaK subunit"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.149

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B3C9 at UniProt or InterPro

Protein Sequence (356 amino acids)

>BWI76_RS13110 phenylacetic acid degradation protein (Klebsiella michiganensis M5al)
MTTFHSLTVAKVEPETRDAVTITFAIPDALRDAYAFRPGQHLTLKARLGGEELRRCYSIC
RSRAPGEISVAVKAIDGGRFSRYAQSDIQPGMALEVMVPQGHFGYQPRAGRRADYLAIAA
GSGITPMMAIVDATLACETDSRFTLIYGNRSSHSMMFRQALADLKDRYPQRLQVVHLFSQ
EAMDSDLLQGRIDGDKLRELAGHLLDFSRFDHAFICGPAAMMDEAEMTLRELGVPEKAIH
LERFNTPGSGARRAAGVQAEGRSVTIRQDGRDRTIALSAEDDSILDAALRQGADLPFACK
GGVCATCKCKVLRGEVAMAANYSLEADEVAAGYVLSCQALPTSGDVIVDFDARGMA