Protein Info for BWI76_RS13080 in Klebsiella michiganensis M5al
Annotation: tyramine oxidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 98% identical to AMO_KLEOK: Primary amine oxidase (maoA) from Klebsiella oxytoca (strain ATCC 8724 / DSM 4798 / JCM 20051 / NBRC 3318 / NRRL B-199 / KCTC 1686)
KEGG orthology group: K00276, primary-amine oxidase [EC: 1.4.3.21] (inferred from 93% identity to kva:Kvar_2900)MetaCyc: 87% identical to copper-containing amine oxidase (Escherichia coli K-12 substr. MG1655)
Primary-amine oxidase. [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]
Predicted SEED Role
"Monoamine oxidase (1.4.3.4)" in subsystem Aromatic Amin Catabolism or Auxin biosynthesis or Glycine and Serine Utilization or Threonine degradation
MetaCyc Pathways
- superpathway of L-threonine metabolism (18/18 steps found)
- superpathway of phenylethylamine degradation (11/11 steps found)
- L-threonine degradation III (to methylglyoxal) (3/3 steps found)
- phenylethylamine degradation I (2/2 steps found)
- putrescine degradation III (2/4 steps found)
- salidroside biosynthesis (2/4 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
- phenylethanol biosynthesis (2/5 steps found)
- L-tryptophan degradation X (mammalian, via tryptamine) (1/4 steps found)
- serotonin degradation (3/7 steps found)
- dopamine degradation (1/5 steps found)
- aromatic biogenic amine degradation (bacteria) (3/8 steps found)
- noradrenaline and adrenaline degradation (4/13 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Drug metabolism - cytochrome P450
- Glycine, serine and threonine metabolism
- Histidine metabolism
- Phenylalanine metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Urea cycle and metabolism of amino groups
- beta-Alanine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.3.21 or 1.4.3.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B3D3 at UniProt or InterPro
Protein Sequence (752 amino acids)
>BWI76_RS13080 tyramine oxidase (Klebsiella michiganensis M5al) MAILSPRKTALALAVALFCAWQSPAFAHGGEAHMVPMDKTLQDFGVDVQWDDYAQMFTLI KDGAYVKVKPGAKTAIVNGKTLELQVPVVMKDGKAWVSDTFINDVFQSGLDQTFQVEKRP HPLNSLSAAEISAAVAIVKAAADFKPNTRFTEISLREPDKKAVWDFALNGTPVNAPRTAD VIMLDGKHVIEAVVDLQNKKVLSWTPIKDAHGMVLLDDFASVQNIINASSEFAEVLKKHG IDDPSKVITTPLTVGYFDGKDGLKQDARLLKVVSYLDVGDGNYWAHPIENLVAVVDLEQK KIIKIEEGPTIPVPMAARPYDGRDRVAPTVKPLEIIEPEGKNYTITGDMIHWRNWDFHLR MNSRVGPILSTVTWNDNGKKRQVMYEGSLGGMIVPYGDPDVGWYFKAYLDSGDYGMGTLT SPIVRGKDAPSNAVLLDETIADYTGTPTTIPRAIAIFERYAGPEYKHLEMGKPNVSTERR ELVVRWISTVGNYDYIFDWVFHENGTIGIDAGATGIEAVKGVQAKTMHDPSAKEDTRYGT LIDHNIVGTTHQHIYNFRLDLDVDGENNTLVAMDPEVKPNTAGGPRTSTMQINQYTIASE QKAAQKFDPGTIRLLSNTTKENRMGNPVSYQIIPYAGGTHPVATGAKFAPDEWIYHRLSF MDKQLWVTRYHPTERFPEGKYPNRSIHDTGLGQYAKDDESLDNHDDVVWITTGTTHVARA EEWPIMPTEWAHALLKPWNFFDETPTLGEKKQ