Protein Info for BWI76_RS13005 in Klebsiella michiganensis M5al

Annotation: thiamine pyrophosphate protein TPP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 15 to 119 (105 residues), 98.9 bits, E=2.6e-32
• PF00205: TPP_enzyme_M
  • amino acids 213 to 336 (124 residues), 54.7 bits, E=1.4e-18
• PF02775: TPP_enzyme_C
  • amino acids 431 to 559 (129 residues), 86.7 bits, E=2.1e-28

Best Hits

KEGG orthology group: K01576, benzoylformate decarboxylase [EC: 4.1.1.7] (inferred from 97% identity to kva:Kvar_2762)

Predicted SEED Role

"Benzoylformate decarboxylase (EC 4.1.1.7)" in subsystem Benzoate degradation (EC 4.1.1.7)

MetaCyc Pathways

• mandelate degradation to acetyl-CoA (14/18 steps found)
• D-phenylglycine degradation (1/3 steps found)
• 4-hydroxymandelate degradation (3/6 steps found)
• mandelate degradation I (2/5 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (23/35 steps found)
• indole-3-acetate degradation II (1/7 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (17/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Toluene and xylene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.7

Sequence Analysis Tools

See A0A285B357 at UniProt or InterPro

Protein Sequence (564 amino acids)

>BWI76_RS13005 thiamine pyrophosphate protein TPP-binding protein (Klebsiella michiganensis M5al)
MSNAITKVQNANARRGGDVLLEVLESEGVEYVFGNPGTTELPFMDALLRKPSIQYVLALQ
EASAVAMADGYAQAAKKPGFLNLHTAGGLGHGMGNLLNAKCSQTPLVVTAGQQDLRHTTT
DPLLLGDLVGMGKTFAKWSQEVTHVDQLPVLVRRAFHDSDAAPKGSVFLSLPMDVMEAMS
CIGIGAPSTIDRNAVAGSLPLLASKLAAFTPGKVALIAGDEIYQSEAASEVVALAEMLAA
DVYGSTWPNRIPYPTAHPLWRGNLSTKATEINRALGQYDAIFALGGKSLITILYTEGQAV
PDQCKVFQLSADAGDLGRTYSSELSVVGDIKSSLRVLLPELEKATANHRSDYQRRFENAV
NEFELSKESLLGQVNEQQSATVITPLVAAFEAARAIGPDVAIVDEAIATSGSLRKSLNSR
RADQYAFLRGGGLGWGMPAAVGYSLGLGRAPVVCFVGDGAAMYSPQALWTAAHEKLPVTF
IVMNNTEYNVLKNFMRSQADYTSAQTERFIAMDLINPAVDYQALGASMGLETRKVLRAGD
IAPAVEAALASGKPNVIEIIISKS