Protein Info for BWI76_RS12950 in Klebsiella michiganensis M5al

Annotation: acriflavin resistance protein AcrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 45 (45 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 42 to 378 (337 residues), 281.4 bits, see alignment E=3.9e-88 PF25917: BSH_RND" amino acids 67 to 208 (142 residues), 66.7 bits, see alignment E=5.3e-22 PF25878: HH_AAEA_pHBA" amino acids 103 to 174 (72 residues), 33.7 bits, see alignment E=1.7e-11 PF25876: HH_MFP_RND" amino acids 107 to 176 (70 residues), 61.6 bits, see alignment E=2.8e-20 PF25944: Beta-barrel_RND" amino acids 214 to 301 (88 residues), 36.1 bits, see alignment E=2.8e-12 PF25954: Beta-barrel_RND_2" amino acids 250 to 303 (54 residues), 28.5 bits, see alignment 5.6e-10 PF25967: RND-MFP_C" amino acids 308 to 369 (62 residues), 42.6 bits, see alignment E=1.9e-14 PF25989: YknX_C" amino acids 314 to 370 (57 residues), 41.9 bits, see alignment E=3e-14

Best Hits

KEGG orthology group: None (inferred from 94% identity to ent:Ent638_1891)

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B398 at UniProt or InterPro

Protein Sequence (391 amino acids)

>BWI76_RS12950 acriflavin resistance protein AcrA (Klebsiella michiganensis M5al)
MSLQKTWGNFHLTVPGVMLLSALLVGCDEGVAQNAAPQAPAVSAADVVVKSISQWDSFNG
RIEAVESVQLRPRVSGYIDKVNYTDGQEVKKGEVLFTIDDRTYRAALEQAQATLARAKTQ
ASLARSEANRTDKLVNTNLVSREEWEQRRAAATQAQADIRAAQAAVDAAQLNLDFTKVTA
PIDGRASRALITSGNLVTAGDSASVLTTLVSQKTVYVYFDVDESTYLHYQNLARSGQGAS
SNHLALPVEIGLVGEEGYPHQGKVDFLDNQLTPSTGTIRMRALLDNAQRQFTPGLFARVR
LPGSAEFNATLIDDKAVLTDQDRKYVYVVDKEGKAQRRDITPGRLADGLRIVQQGLKPGD
KVIVDGLQKVFMPGMPVNAKTVAMTASTALN