Protein Info for BWI76_RS11925 in Klebsiella michiganensis M5al

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 865 TIGR01051: DNA topoisomerase I" amino acids 5 to 635 (631 residues), 867.3 bits, see alignment E=4.8e-265 PF01751: Toprim" amino acids 5 to 142 (138 residues), 76.6 bits, see alignment E=3.4e-25 PF01131: Topoisom_bac" amino acids 157 to 564 (408 residues), 431.6 bits, see alignment E=8.4e-133 PF01396: zf-C4_Topoisom" amino acids 597 to 622 (26 residues), 25.6 bits, see alignment (E = 2.2e-09) amino acids 709 to 745 (37 residues), 44.5 bits, see alignment (E = 2.7e-15) PF21372: TOP1_ZnF" amino acids 651 to 695 (45 residues), 99.5 bits, see alignment 1.7e-32 PF08272: Topo_Zn_Ribbon" amino acids 758 to 793 (36 residues), 44.7 bits, see alignment (E = 2.2e-15) amino acids 824 to 862 (39 residues), 76.5 bits, see alignment (E = 2.4e-25)

Best Hits

Swiss-Prot: 93% identical to TOP1_SALTI: DNA topoisomerase 1 (topA) from Salmonella typhi

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 94% identity to cro:ROD_17291)

MetaCyc: 93% identical to DNA topoisomerase I (Escherichia coli K-12 substr. MG1655)
5.99.1.2-RXN [EC: 5.6.2.1]

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.6.2.1 or 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B2S6 at UniProt or InterPro

Protein Sequence (865 amino acids)

>BWI76_RS11925 DNA topoisomerase I (Klebsiella michiganensis M5al)
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTSTKGAKKPK
KDERGALVNRMGVDPWHDWDAHYEILPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA
WHLREVIGGDEQRYSRVVFNEITKNAIRQAFEKPGELNIDRVNAQQARRFMDRVVGYMVS
PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEYWEVDASTTTPGGDALPLQVTH
SGDKPFRPVSRDETMAAVALLEKASYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFG
VKKTMMMAQRLYEAGHITYMRTDSTNLSQDALNMVRGYIGDNFGKKYLPESANQYASKEN
SQEAHEAIRPSDVNVLAETLKDMESDAQKLYQLIWRQFVACQMTPAQYDSTTLTVKAGDF
KLKARGRTLRFDGWTKVMPALRKGDEDRTLPLVNKGESLSLVELTPAQHFTKPPARFSEA
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRDLMNY
DFTAQMEDRLDQVANHQAEWKQVLDNFFGDFTSQLQTAEKDPEEGGMQPNPMVLTTIDCP
TCGRKMGIRTASTGVFLGCSGYALSPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR
RCKKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL
KMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF
LAANTFPKSRETRAPLVEELYRFRDRLAEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV
ASEKEGKATGWSAFYIDGKWTEAKK