Protein Info for BWI76_RS11890 in Klebsiella michiganensis M5al

Annotation: glycosyl hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 785 PF00933: Glyco_hydro_3" amino acids 99 to 316 (218 residues), 148.5 bits, see alignment E=5.2e-47 PF01915: Glyco_hydro_3_C" amino acids 347 to 582 (236 residues), 170.4 bits, see alignment E=7.6e-54 PF14310: Fn3-like" amino acids 619 to 689 (71 residues), 69.1 bits, see alignment E=4.5e-23

Best Hits

KEGG orthology group: None (inferred from 87% identity to eae:EAE_21380)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B2R7 at UniProt or InterPro

Protein Sequence (785 amino acids)

>BWI76_RS11890 glycosyl hydrolase (Klebsiella michiganensis M5al)
MSNDFNTTLAAMSAEEKVALLTGSGLWRTASLPQHGIEDIIMTDGTYGVRYSSAQIDGRE
KWSMDDFISVITQTADQASVAEPAAKGGSEALFSTSLPATCFPNGSSLACSWDVELVHQM
GQALGRECQQMGVGILLGPGINIRRTPLAGRGYEYYAEDPVVSGDIAAALINGLQQEGVG
ASLKHFAANNSEFRRTEMDSIIEERALREIYLAGFQRAIAKSRPWTVMSSYNRLNGVQTS
QDPFLLTQVLRDEWGYDGLVMSDWYGIKDRPASLMAGNDLAMPETRRDKQTLLAAIEAEE
VPMAVVDRACLRMLELLDKVQRHRRPQTRADFTAHHALAQRLAGESIVLLKNEEALLPLR
SEKTPRIAVLGKPAQEPVIQGSGCATTVPYLLDRPLDEIFDLAGDDFSVTWAVGAPDDLR
DDEQALAQACSVAQEADVAVLFVSTAVGEDGENGDRQDLHILPVHERLIREVAAVQPNIV
VVLANSDAVVMPWLGECKALLETFFAGQGMGRAVAEILFGKRNPCGKLTVTVPNSLEETP
AWLHYPGENLRHYYGEGLFVGYRYYDKRRLMPQFPFGFGLSYTRFAYDNIALSASRLGAD
ETLTVSFDLTNCGEYAGKEIVQLYVSAPKGELIREVQSLKAFSKVALAAGESRRVSLQLP
IAELACYHPGLGDWMVTPGIWQIRVGASSRDLPLTAEMEVDCPQRYVPLRDDNSLQQLIQ
QPEAFARVVTLIADKSQIPAEQVREKLIRLAPDLFCGLLIALTEFLALDIERDELNAVLA
GAHHC