Protein Info for BWI76_RS11855 in Klebsiella michiganensis M5al

Annotation: bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00566: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
  • amino acids 4 to 186 (183 residues), 179 bits, E=8.8e-57
• PF00117: GATase
  • amino acids 5 to 186 (182 residues), 173.5 bits, E=8.3e-55
• PF07722: Peptidase_C26
  • amino acids 75 to 172 (98 residues), 23.5 bits, E=9e-09
• PF02885: Glycos_trans_3N
  • amino acids 201 to 263 (63 residues), 59.8 bits, E=3.6e-20
• TIGR01245: anthranilate phosphoribosyltransferase
  • amino acids 204 to 528 (325 residues), 426.1 bits, E=1e-131
• PF00591: Glycos_transf_3
  • amino acids 273 to 521 (249 residues), 343.3 bits, E=2e-106

Best Hits

Swiss-Prot: 94% identical to TRPGD_ECOLI: Bifunctional protein TrpGD (trpGD) from Escherichia coli (strain K12)

KEGG orthology group: K13497, anthranilate synthase/phosphoribosyltransferase [EC: 2.4.2.18 4.1.3.27] (inferred from 97% identity to kpe:KPK_3180)

MetaCyc: 94% identical to anthranilate synthase subunit TrpD (Escherichia coli K-12 substr. MG1655)
Anthranilate synthase. [EC: 4.1.3.27]; Anthranilate phosphoribosyltransferase. [EC: 4.1.3.27, 2.4.2.18]

Predicted SEED Role

"Anthranilate synthase, amidotransferase component (EC 4.1.3.27) / Anthranilate phosphoribosyltransferase (EC 2.4.2.18)" in subsystem Auxin biosynthesis or Tryptophan synthesis or Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. (EC 2.4.2.18, EC 4.1.3.27)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
• acridone alkaloid biosynthesis (1/4 steps found)
• superpathway of candicidin biosynthesis (3/11 steps found)
• superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
• chloramphenicol biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.18, 4.1.3.27

Use Curated BLAST to search for 2.4.2.18 or 4.1.3.27

Sequence Analysis Tools

See A0A285B2J4 at UniProt or InterPro

Protein Sequence (531 amino acids)

>BWI76_RS11855 bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase (Klebsiella michiganensis M5al)
MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNSVPAQALIERIGTMKNPVLMLSPGPGT
PSEAGCMPELLIRMRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
AGLSNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGA
RLLEQTLAWALQKLEQTNTLQPILEKLYQAETLSQQESHQLFSAVVRGEVKPEQLAAALV
SMKVRGEHPQEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHA
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
MDEVSLHAPTVVAELNNGEIKSYQLTAADFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
EAAHEAAVAANVAMLMRLHGYEDLKANAQQVIDVLHSGAAYDRVTALAARG