Protein Info for BWI76_RS11775 in Klebsiella michiganensis M5al
Annotation: 2-deoxy-D-gluconate 3-dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to KDUD_ECOLI: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD) from Escherichia coli (strain K12)
KEGG orthology group: K00065, 2-deoxy-D-gluconate 3-dehydrogenase [EC: 1.1.1.125] (inferred from 87% identity to ent:Ent638_1716)MetaCyc: 76% identical to putative 2-keto-3-deoxy-D-gluconate dehydrogenase (Escherichia coli K-12 substr. MG1655)
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase. [EC: 1.1.1.127]; 1.1.1.- [EC: 1.1.1.127]; RXN0-7101 [EC: 1.1.1.127]
Predicted SEED Role
"2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.125)
MetaCyc Pathways
- 4-deoxy-L-threo-hex-4-enopyranuronate degradation (5/5 steps found)
- 2-deoxy-D-glucose 6-phosphate degradation (3/4 steps found)
- 3,6-anhydro-α-L-galactopyranose degradation (4/7 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (19/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.125
Use Curated BLAST to search for 1.1.1.125 or 1.1.1.127
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B2P6 at UniProt or InterPro
Protein Sequence (253 amino acids)
>BWI76_RS11775 2-deoxy-D-gluconate 3-dehydrogenase (Klebsiella michiganensis M5al) MVLNAFDLAGKVAIVTGCDTGLGQGMTLGLAQAGCDIVGVNRRIPHETAEKVQALGRRFT AIQADLSRQDEIEDIVTQAVAAMGRIDILVNNAGTIRRADALSFSEKDWDDVMNLNLKSV FFLSQAVARQFIQQGDGGKIVNIASMLSFQGGIRVPSYTASKSGVLGITRLMANEWAGHR INVNAIAPGYMATNNTQQLRDDAERSKAILDRIPAGRWGVADDLQGPVVFLASRAADYIS GYTLAVDGGWLAR