Protein Info for BWI76_RS11670 in Klebsiella michiganensis M5al
Updated annotation (from data): Succinylornithine transaminase (EC 2.6.1.81)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCORNTRANSAM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: aspartate aminotransferase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to ASTC_ESCF3: Succinylornithine transaminase (astC) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
KEGG orthology group: None (inferred from 90% identity to eae:EAE_21600)MetaCyc: 82% identical to succinylornithine transaminase (Escherichia coli K-12 substr. MG1655)
Succinylornithine transaminase. [EC: 2.6.1.81]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.11]
Predicted SEED Role
"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)
MetaCyc Pathways
- superpathway of arginine and polyamine biosynthesis (16/17 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-arginine degradation II (AST pathway) (5/5 steps found)
- L-ornithine biosynthesis I (5/5 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Lysine biosynthesis
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.81
Use Curated BLAST to search for 2.6.1.11 or 2.6.1.81
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B2H7 at UniProt or InterPro
Protein Sequence (406 amino acids)
>BWI76_RS11670 Succinylornithine transaminase (EC 2.6.1.81) (Klebsiella michiganensis M5al) MSQSITRSNFDEWMMPVYAPAAFIPVRGEGSRLWDQQGKEYIDFAGGIAVNALGHAHPRL VKALTEQAGKFWHTGNGYTNEPVLRLAKQLIDATFADRVFFCNSGAEANEAALKLARKYA HDRFGSEKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAPLPPQIQHAIYNDLDSAKALI DDNTCAVIVEPMQGEGGVVPADADFLRGLRELCDAHNALLIFDEVQTGVGRTGELYAYMH YGVTPDLLSTAKALGGGFPIGALLASERCASVMTVGTHGTTYGGNPLACAVAGEVFATIN TREVLNGVKQRHQWFCERLNAINARYGLFKEIRGLGLLIGCVLKDEYAGKAKAISNQAAE EGLMILIAGANVVRFAPALIISEDEVNSGLDRFELACKRFLAGVSS