Protein Info for BWI76_RS11630 in Klebsiella michiganensis M5al

Annotation: sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00581: Rhodanese
  • amino acids 179 to 264 (86 residues), 42.4 bits, E=4e-15
  • amino acids 297 to 418 (122 residues), 46.5 bits, E=2.1e-16

Best Hits

Swiss-Prot: 80% identical to YNJE_ECOLI: Thiosulfate sulfurtransferase YnjE (ynjE) from Escherichia coli (strain K12)

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 87% identity to kpu:KP1_2242)

MetaCyc: 80% identical to molybdopterin synthase sulfurtransferase (Escherichia coli K-12 substr. MG1655)
RXN-12473 [EC: 2.8.1.11]

Predicted SEED Role

"Putative thiosulfate sulfurtransferase ynjE (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

• molybdopterin biosynthesis (5/6 steps found)
• L-cysteine degradation III (2/2 steps found)
• thiosulfate disproportionation IV (rhodanese) (1/1 steps found)
• sulfide oxidation IV (mitochondria) (2/5 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.11 or 2.8.1.2

Sequence Analysis Tools

See A0A285B2N6 at UniProt or InterPro

Protein Sequence (435 amino acids)

>BWI76_RS11630 sulfurtransferase (Klebsiella michiganensis M5al)
MKRVSQLTALALLMGLAAAATSAADAMPTLTLSTLQQHNGVAIDTRLSAFYNGWPQGANG
PQGHEPQALNLAAGWLGAMTDEQLGAWAKLHNLQASTPIALYGDADDNAKVAARLKAAGF
THLSSLGDALSQAERLQKLPHFEQLVYPQWLHDLQQGKAVAAAPAGKWKVFEAAWGAPKL
YLLSHIPGAGYIDTNEVESEPLWNKVSDAQLKAMLAKHGIRHDTTVILYGRDVYAAARVA
QIMLYAGVKDVRLLDGGWKTWSAAGLPVERGTPPKQKPEPEFGAAIPGQPQLMLNTEQAR
ALLHRQDASLVSIRSWPEFIGTTSGYSYIKPMGEIAGARWGHAGSDSTHMEDFHNPDGTM
RSADDIATMWKSWNILPSQQVAFYCGTGWRASETFMYARAMGWNNVSVYDGGWYEWSSNP
KNPVSRGERGPESSK