Protein Info for BWI76_RS11395 in Klebsiella michiganensis M5al

Annotation: RNA polymerase sigma factor FecI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 173 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 15 to 164 (150 residues), 71 bits, see alignment E=4.5e-24 PF04542: Sigma70_r2" amino acids 16 to 81 (66 residues), 47.4 bits, see alignment E=2.6e-16 PF07638: Sigma70_ECF" amino acids 42 to 160 (119 residues), 24.2 bits, see alignment E=5.7e-09 PF08281: Sigma70_r4_2" amino acids 112 to 164 (53 residues), 58.5 bits, see alignment E=8.1e-20 PF04545: Sigma70_r4" amino acids 118 to 162 (45 residues), 26 bits, see alignment E=1e-09

Best Hits

Swiss-Prot: 69% identical to FECI_ECOLI: Probable RNA polymerase sigma factor FecI (fecI) from Escherichia coli (strain K12)

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 71% identity to eca:ECA1079)

MetaCyc: 69% identical to RNA polymerase sigma factor FecI (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor FecI"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B2B1 at UniProt or InterPro

Protein Sequence (173 amino acids)

>BWI76_RS11395 RNA polymerase sigma factor FecI (Klebsiella michiganensis M5al)
MSPSSAIASPLTLASLYSDHHGWLKKWLTHKLKSVYDADDVAQDTFVRIMAGGSLSTIRD
PKSFLCTIANRVMIDLFRRNALERAWLEMLSQLPEELSPSPEQRQSQLELLQQIDAMLDG
LNVKAREAFLLSQLEGLTYADIASRLAVSVSSVKKYIAKATEHCLLFRLENGL