Protein Info for BWI76_RS11320 in Klebsiella michiganensis M5al

Annotation: NUDIX hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00293: NUDIX
  • amino acids 4 to 116 (113 residues), 67.6 bits, E=5.7e-23

Best Hits

Swiss-Prot: 81% identical to NUDJ_ECO24: Phosphatase NudJ (nudJ) from Escherichia coli O139:H28 (strain E24377A / ETEC)

KEGG orthology group: None (inferred from 91% identity to eae:EAE_16580)

MetaCyc: 81% identical to phosphatase NudJ (Escherichia coli K-12 substr. MG1655)
RXN0-7309 [EC: 3.6.1.76]; Guanosine-diphosphatase. [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]; 3.6.1.- [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]; 3.6.1.- [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]

Predicted SEED Role

"Nudix-like NDP and NTP phosphohydrolase YmfB" in subsystem Nudix proteins (nucleoside triphosphate hydrolases)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.42 or 3.6.1.5 or 3.6.1.76

Sequence Analysis Tools

See A0A285B2F1 at UniProt or InterPro

Protein Sequence (158 amino acids)

>BWI76_RS11320 NUDIX hydrolase (Klebsiella michiganensis M5al)
MFKPHVTVACVVHAKGKFLVVEETINGKVLWNQPAGHLEADETLVQAAERELWEETGIRA
TPQHFLRLHQWIAPDRTPFLRFLFTIELNDTCATEPHDSDIDRCLWLSAEEILNATNLRS
QLVAESIRSYQQDPRYPLSLIGEFNWPFTGGASTEDAC