Protein Info for BWI76_RS11315 in Klebsiella michiganensis M5al

Annotation: tRNA 2-thiouridine(34) synthase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 PF02568: ThiI" amino acids 4 to 75 (72 residues), 30.8 bits, see alignment E=5.5e-11 PF02540: NAD_synthase" amino acids 4 to 75 (72 residues), 29.5 bits, see alignment E=1e-10 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 6 to 360 (355 residues), 525.1 bits, see alignment E=3.7e-162 PF03054: tRNA_Me_trans" amino acids 6 to 202 (197 residues), 282.1 bits, see alignment E=6.4e-88 PF20259: tRNA_Me_trans_M" amino acids 208 to 275 (68 residues), 81.7 bits, see alignment E=5.6e-27 PF20258: tRNA_Me_trans_C" amino acids 286 to 360 (75 residues), 96 bits, see alignment E=3.4e-31

Best Hits

Swiss-Prot: 95% identical to MNMA_KLEP3: tRNA-specific 2-thiouridylase MnmA (mnmA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_16575)

MetaCyc: 94% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B294 at UniProt or InterPro

Protein Sequence (368 amino acids)

>BWI76_RS11315 tRNA 2-thiouridine(34) synthase MnmA (Klebsiella michiganensis M5al)
MSESQKKVIVGMSGGVDSSVSAYLLLQQGYKVEGLFMKNWEEDDGEEYCTAAADLADAQA
VCDKLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLG
ADYIATGHYVRRADVNGKSQLLRGLDGNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRK
IAEELDLITAKKKDSTGICFIGERKFREFLGRYLPAQPGKIISVDGDEVGEHQGLMYHTL
GQRKGLGIGGIKDGGEDPWYVVDKDVENNILVVAQGHDHPRLMSVGLIAQQLHWVDREPV
TGTLRCTVKTRYRQTDIPCSVKALDDDRIEVIFDEPVAAVTPGQSAVFYRGEVCLGGGII
EQRLPLPA