Protein Info for BWI76_RS11160 in Klebsiella michiganensis M5al

Annotation: thiamine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 64% identical to THIK_SALDC: Thiamine kinase (thiK) from Salmonella dublin (strain CT_02021853)

KEGG orthology group: K07251, thiamine kinase [EC: 2.7.1.89] (inferred from 76% identity to kva:Kvar_3277)

MetaCyc: 63% identical to thiamine kinase (Escherichia coli K-12 substr. MG1655)
Thiamine kinase. [EC: 2.7.1.89]

Predicted SEED Role

"Thiamine kinase (EC 2.7.1.89) @ Adenosylcobinamide kinase (EC 2.7.1.156)" (EC 2.7.1.156, EC 2.7.1.89)

MetaCyc Pathways

superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
thiamine diphosphate salvage I (2/2 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.156

Use Curated BLAST to search for 2.7.1.156 or 2.7.1.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B257 at UniProt or InterPro

Protein Sequence (272 amino acids)

>BWI76_RS11160 thiamine kinase (Klebsiella michiganensis M5al)
MQFSNNKLTRDEVLSRFFPRYRPVAALSQNGLSGGSCILSDGRQRRVLRQPHDPHAPACD
FRRQYHVLSRLPDSLAPAPVFYSPAWMVVEYCAGDVVNALPECPALAGLLYHLHRQPRFG
WRIALRPLLELYWQQCSPARRTPRWLRWHKRLLRRGEPRPLRLAPLHMDVHAGNIVHGEA
GLRLIDWEYAGDGDIALELAAVWINPAAHRRLAAEYARQASIDEHQLWRQIQRWRPWVRL
LMAGWYERRWQQTGDRQFIALADEIWRQLDKK