Protein Info for BWI76_RS11120 in Klebsiella michiganensis M5al

Annotation: DNA polymerase III subunit delta'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 PF13177: DNA_pol3_delta2" amino acids 11 to 166 (156 residues), 129.2 bits, see alignment E=2.3e-41 TIGR00678: DNA polymerase III, delta' subunit" amino acids 12 to 200 (189 residues), 193 bits, see alignment E=1.9e-61 PF21500: HolB_lid" amino acids 167 to 206 (40 residues), 68.1 bits, see alignment 7.1e-23 PF09115: DNApol3-delta_C" amino acids 209 to 321 (113 residues), 117.8 bits, see alignment E=5.7e-38

Best Hits

Swiss-Prot: 72% identical to HOLB_ECOLI: DNA polymerase III subunit delta' (holB) from Escherichia coli (strain K12)

KEGG orthology group: K02341, DNA polymerase III subunit delta' [EC: 2.7.7.7] (inferred from 84% identity to kpn:KPN_01097)

MetaCyc: 72% identical to DNA polymerase III subunit delta' (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"DNA polymerase III delta prime subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B293 at UniProt or InterPro

Protein Sequence (334 amino acids)

>BWI76_RS11120 DNA polymerase III subunit delta' (Klebsiella michiganensis M5al)
MKWYSWLRPPFEQLVASYQAGRGHHALMVQALPGMGSDALIYALCRFLMCRQPEGHKSCG
HCHSCQLMQASTHPDYYALTPEKGKSSLGIDAVREVSEKLYEHARLGGAKVVWIGDAAML
TDAAANALLKTLEEPPENTWFFLACQEPARLLATLRSRCRLFHLAPPSERYALAWLEREV
TMSQDALLAALRLSANAPAAALDLLQEAHWAPRQQLCQILGNILTHGDWLTLLPMLNHEQ
AAIRLHWLASLLVDAQKRQQGITLISNPDVWPLVNQLAAALPAARLQAIARDVSACREQL
LSVVGINRELLLTERLLRWEHYLQPGAVLPVSHL