Protein Info for BWI76_RS10795 in Klebsiella michiganensis M5al

Updated annotation (from data): L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2)
Rationale: Specifically important for utilizing L-Proline. Automated validation from mutant phenotype: the predicted function (1.2.1.88, 1.5.5.2) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 76.1 bits, see alignment (E = 2.7e-25)
• PF18327: PRODH
  • amino acids 89 to 136 (48 residues), 56.2 bits, see alignment (E = 8.4e-19)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 147 to 259 (113 residues), 153.8 bits, E=5.7e-49
• PF01619: Pro_dh
  • amino acids 268 to 569 (302 residues), 356.5 bits, E=3e-110
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 610 to 1113 (504 residues), 905.3 bits, E=4.4e-277
• PF00171: Aldedh
  • amino acids 659 to 1105 (447 residues), 362.4 bits, E=7.7e-112

Best Hits

Swiss-Prot: 95% identical to PUTA_KLEAE: Bifunctional protein PutA (putA) from Klebsiella aerogenes

MetaCyc: 90% identical to fused DNA-binding transcriptional repressor / proline dehydrogenase / 1-pyrroline-5-carboxylate dehydrogenase PutA (Escherichia coli K-12 substr. MG1655)
RXN0-7008 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-proline degradation I (3/3 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• proline to cytochrome bo oxidase electron transfer (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)
• L-arginine degradation I (arginase pathway) (1/3 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Sequence Analysis Tools

See A0A285B255 at UniProt or InterPro

Protein Sequence (1320 amino acids)

>BWI76_RS10795 L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (Klebsiella michiganensis M5al)
MGTTTMGVKLDDATRERIKSAASRIDRTPHWLIKQAIFNYLEKLENDETLPELPALLSGA
ANESDEAGNPADEPYQPFLDFAEQILPQSVSRASITAAWRWAETDAVPMLLEQARLPQTL
GEQAHKLAYQLAEKLRNQKTASGRAGMVQSLLQEFSLSSQEGVALMCLAEALLRIPDKAT
RDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGKLVSPHNETSLSRSLNRIIGKSG
EPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLEDKGFRYSYDMLGEAALTADDAQAY
MVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRAMEELYPRLKSLTLLA
RQYDIGINIDAEEADRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPFVIDYLIDL
ATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLI
YPQFATHNAHTLAAIYQLAGQNYYPGQYEFQCLHGMGEPLYEQVTGKIADGKLNRPCRIY
APVGTHETLLAYLVRRLLENGANTSFVNRIADSTLPLDELVADPVAAVEKLAQQEGQVGL
PHPKIPLPRDLYGKGRSNSAGLDLANEHRLASLSSSLLNSALHKWQALPMLEQPVAEGEM
QPVINPAEPKDIVGYVREASDDEVQQALTSAVNNAPIWFATPPQERAAILERAAVLMEGQ
MPTLMGILVREAGKTFSNAIAEVREAVDFLHYYAGQVRNDFDNETHRPLGPVVCISPWNF
PLAIFSGQIAAALAAGNTVLAKPAEQTPLIAAQGVAILLEAGVPPGVIQLLPGRGETVGA
ALTSDERVRGVMFTGSTEVATLLQRNIASRLDAQGRPTPLIAETGGMNAMIVDSSALTEQ
VVIDVLASAFDSAGQRCSALRVLCLQDDIADHTLTMLRGAMAECRMGNPGRLTTDIGPVI
DAEAKENIERHIQTLRAKGRKVFQAVRENGEDSREWASGTFVPPTLIELDSFDELKKEVF
GPVLHVVRYNRNELEGLVEQINASGYGLTLGVHTRIDETIAQVTGSANVGNLYVNRNMVG
AVVGVQPFGGEGLSGTGPKAGGPLYLYRLLSSRPQNAVGITLARQDPEYPLDAQLKTLLE
KPLVALQQWAADRPELQALCQQFSKQAQAGTQRLLPGPTGERNTLTFMPRDRVLCVADNE
QDALTQLAGVTAVGCEVLWPDAPLQRELAKKLPREVSERIHFAKAETLTTEPFDAVIYHG
DSDQLRELCEQVAARDGAIVSVQGFARGESNLLLERLYIERSLSVNTAAAGGNASLMTIG